Carbohydrate Specificity of Antibodies against Phytopathogenic Fungi of the Aspergillus Genus

Carbohydrate Specificity of Antibodies against Phytopathogenic Fungi of the Aspergillus Genus Научная публикация

Журнал

Applied Biochemistry and Microbiology
ISSN: 1608-3024 , E-ISSN: 0003-6838

Вых. Данные

Год: 2018, Том: 54, Номер: 5, Страницы: 522-527 Страниц : 6 DOI: 10.1134/s0003683818050095

Авторы

Krylov V.B. ¹ , Petruk M.I. ¹ , Glushko N.I. ² , Khaldeeva E.V. ² , Mokeeva V.L. ³ , Bilanenko E.N. ³ , Lebedin Y.S. ⁴ , Eremin S.A. ³ , Nifantiev N.E. ¹

Организации

1	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia
2	Kazan Scientific Research Institute of Epidemiology and Microbiology, Kazan, 420015, Russia
3	M.V. Lomonosov State University, Moscow, 119991, Russia
4	XEMA Company Limited, Moscow, 105264, Russia

Brush rabbits were immunized with injections prepared from the fungi Aspergillus fumigatus, Aspergillus niger, and Aspergillus repens. A library of synthetic biotinylated oligosaccharides containing the key fragments of antigenic polysaccharides of the fungal cell wall—galctomannan, α- and β-glucans, mannan, and chitin—was used to analyze carbohydrate specificity. The anticarbohydrate antibodies obtained from animals immunized with preparations from A. fumigatus and A. repens predominantly recognized epitopes containing galactofuranoside residues, while the majority of the antibodies against A. niger bound the chitooligosaccharide ligand. These results are the basis for the identification of specific markers required for the development of immunoenzyme test systems.

Krylov V.B. , Petruk M.I. , Glushko N.I. , Khaldeeva E.V. , Mokeeva V.L. , Bilanenko E.N. , Lebedin Y.S. , Eremin S.A. , Nifantiev N.E.
Carbohydrate Specificity of Antibodies against Phytopathogenic Fungi of the Aspergillus Genus
Applied Biochemistry and Microbiology. 2018. V.54. N5. P.522-527. DOI: 10.1134/s0003683818050095 WOS Scopus OpenAlex

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