Friunavirus Phage-Encoded Depolymerases Specific to Different Capsular Types of Acinetobacter baumannii

Friunavirus Phage-Encoded Depolymerases Specific to Different Capsular Types of Acinetobacter baumannii Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2023, Volume: 24, Number: 10, Article number : 9100, Pages count : DOI: 10.3390/ijms24109100

Authors

Timoshina Olga Y. ^1,2 , Kasimova Anastasia A. ³ , Shneider Mikhail M. ¹ , Matyuta Ilya O. ^4,5 , Nikolaeva Alena Y. ⁶ , Evseev Peter V. ¹ , Arbatsky Nikolay P. ³ , Shashkov Alexander S. ³ , Chizhov Alexander O. ³ , Shelenkov Andrey A. ⁷ , Mikhaylova Yulia V. ⁷ , Slukin Pavel V. ² , Volozhantsev Nikolay V. ² , Boyko Konstantin M. ⁴ , Knirel Yuriy A. ³ , Miroshnikov Konstantin A. ¹ , Popova Anastasia V. ²

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
2	State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia
3	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
4	Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia
5	Center for Photonics and 2D Materials, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia
6	Complex of NBICS Technologies, National Research Center “Kurchatov Institute”, 123182 Moscow, Russia
7	Central Scientific Research Institute of Epidemiology, 111123 Moscow, Russia

Acinetobacter baumannii is a critical priority nosocomial pathogen that produces a variety of capsular polysaccharides (CPSs), the primary receptors for specific depolymerase-carrying phages. In this study, the tailspike depolymerases (TSDs) encoded in genomes of six novel Friunaviruses, APK09, APK14, APK16, APK86, APK127v, APK128, and one previously described Friunavirus phage, APK37.1, were characterized. For all TSDs, the mechanism of specific cleavage of corresponding A. baumannii capsular polysaccharides (CPSs) was established. The structures of oligosaccharide fragments derived from K9, K14, K16, K37/K3-v1, K86, K127, and K128 CPSs degradation by the recombinant depolymerases have been determined. The crystal structures of three of the studied TSDs were obtained. A significant reduction in mortality of Galleria mellonella larvae infected with A. baumannii of K9 capsular type was shown in the example of recombinant TSD APK09_gp48. The data obtained will provide a better understanding of the interaction of phage–bacterial host systems and will contribute to the formation of principles of rational usage of lytic phages and phage-derived enzymes as antibacterial agents.

Timoshina O.Y. , Kasimova A.A. , Shneider M.M. , Matyuta I.O. , Nikolaeva A.Y. , Evseev P.V. , Arbatsky N.P. , Shashkov A.S. , Chizhov A.O. , Shelenkov A.A. , Mikhaylova Y.V. , Slukin P.V. , Volozhantsev N.V. , Boyko K.M. , Knirel Y.A. , Miroshnikov K.A. , Popova A.V.
Friunavirus Phage-Encoded Depolymerases Specific to Different Capsular Types of Acinetobacter baumannii
International Journal of Molecular Sciences. 2023. V.24. N10. 9100 . DOI: 10.3390/ijms24109100 WOS Scopus OpenAlex

Web of science:	WOS:000997489800001
Scopus:	2-s2.0-85160373335
OpenAlex:	W4378082911

DB	Citing
OpenAlex	13
Scopus	10
Web of science	13