Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside Научная публикация

Журнал

Beilstein Journal of Organic Chemistry
ISSN: 2195-951X , E-ISSN: 1860-5397

Вых. Данные

Год: 2020, Том: 11, Страницы: 480-493 Страниц : 14 DOI: 10.3762/bjnano.11.39

Авторы

Burygin Gennady L ^1,2 , Abronina Polina I ³ , Podvalnyy Nikita M ³ , Staroverov Sergey A ² , Kononov Leonid O ^3,4 , Dykman Lev A ²

Организации

1	Department of Horticulture, Breeding, and Genetics, Vavilov Saratov State Agrarian University, Teatralnaya Ploshchad 1, Saratov, 410012, Russia,
2	Laboratory of Immunochemistry, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, Saratov, 410049, Russia,
3	Laboratory of Carbohydrate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospekt 47, Moscow, 119991, Russia and
4	Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (National Research University), Institutsky per. 9, Dolgoprudnyi, Moscow Region, 141701, Russia

A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we aimed to prepare and evaluate in vivo glyco-gold nanoparticles (glyco-GNPs) bearing the terminal-branched hexaarabinofuranoside fragment (Ara6) of arabinan domains of lipoarabinomannan and arabinogalactan, which are principal polysaccharides of the cell wall of Mycobacterium tuberculosis, the causative agent of tuberculosis. In particular, we were interested whether the antibodies generated against Ara6-GNPs would recognize the natural saccharides on the cell surface of different mycobacterial strains. Two synthetic Ara6 glycosides with amino-functionalized spacer aglycons differing in length and hydrophilicity were directly conjugated with spherical gold nanoparticles (d = 15 nm) to give two sets of glyco-GNPs, which were used for the immunization of rabbits. Dot assays revealed cross-reactions between the two obtained antisera with the hexaarabinofuranoside and the 2-aminoethyl aglycon used for the preparation of glyco-GNPs. Both antisera contained high titers of antibodies specific for Mycobacteria as shown by enzyme-linked immunosorbent assay using M. bovis and M. smegmatis cells as antigens while there was only a weak response to M. phlei cells and no interaction with E. coli cells. The results obtained suggest that glyco-GNPs are promising agents for the generation of anti-mycobacterial antibodies.

Burygin G.L. , Abronina P.I. , Podvalnyy N.M. , Staroverov S.A. , Kononov L.O. , Dykman L.A.
Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside
Beilstein Journal of Organic Chemistry. 2020. V.11. P.480-493. DOI: 10.3762/bjnano.11.39 WOS Scopus OpenAlex

Web of science:	WOS:000520020800001
Scopus:	2-s2.0-85084182975
OpenAlex:	W3012105800

БД	Цитирований
OpenAlex	27
Scopus	26
Web of science	24