Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage

Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage Научная публикация

Журнал

Russian Chemical Bulletin
ISSN: 1573-9171 , E-ISSN: 1066-5285

Вых. Данные

Год: 2011, Том: 60, Номер: 4, Страницы: 746-753 Страниц : 8 DOI: 10.1007/s11172-011-0115-x

Авторы

Krylov V.B. ¹ , Grachev A.A. ¹ , Ustyuzhanina N.E. ¹ , Ushakova N.A. ² , Preobrazhenskaya M.E. ² , Kozlova N.I. ² , Portsel M.N. ³ , Konovalova I.N. ³ , Novikov V.Yu. ⁴ , Siebert H.Ch. ^5,6 , Shashkov A.S. ¹ , Nifantiev N.E. ¹

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky prosp., 119991, Moscow, Russian Federation
2	V. N. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 ul. Pogodinskaya, 119121, Moscow, Russian Federation
3	Murmansk State Technical University, 13 ul. Sportivnaya, 183010, Murmansk, Russian Federation
4	N. M. Knipovich Polar Research Institute of Marine Fisheries and Oceanography, 6 ul. Knipovicha, 183000, Murmansk, Russian Federation
5	RI-B-NT Research Institute of Bioinformatics and Nanotechnology, Schauenburgerstrasse 116, D-24118, Kiel, Germany
6	Institute for Biochemistry and Endocrinology, Veterinary Faculty, Justus Liebig University. Frankfurter Strasse 100, D35392 Giessen, Germany

Chondroitin sulfates isolated from cartilage of five marine fish species: Atlantic salmon (Salmo salar), Greenland shark (Somniosus microcephalus), blackmouth catshark (Galeus melastomus), birdbeak dogfish (Deania calcea), and Arctic skate (Amblyraja hyperborea), were characterized in detail by 1H and 13C NMR spectroscopy. The complete signal assignments for carbohydrates were made and the relative contents of the key structural units were estimated by 2D homonuclear and heteronuclear NMR spectroscopy (COSY, TOCSY, NOESY, HSQC, and HMBC). The average length of the polysaccharide chain was evaluated from the integrated intensity ratio of the terminal and internal monosaccharide residues. The anti-inflammatory and anticoagulant activities of the specimens were studied. Chondroitin sulfates from salmon and Arctic skate exhibit considerable anti-inflammatory activity. All specimens manifest weak anticoagulant activity. The results of the present study indicate that chondroitin sulfates deserve more detailed investigation as potential anti-inflammatory agents.

Krylov V.B. , Grachev A.A. , Ustyuzhanina N.E. , Ushakova N.A. , Preobrazhenskaya M.E. , Kozlova N.I. , Portsel M.N. , Konovalova I.N. , Novikov V.Y. , Siebert H.C. , Shashkov A.S. , Nifantiev N.E.
Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage
Russian Chemical Bulletin. 2011. V.60. N4. P.746-753. DOI: 10.1007/s11172-011-0115-x WOS Scopus OpenAlex

≡ Web of science:	WOS:000297199600020
≡ Scopus:	2-s2.0-83455229403
≡ OpenAlex:	W2011815227