Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis

Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2021, Volume: 193, Pages: 1294-1300 Pages count : 7 DOI: 10.1016/j.ijbiomac.2021.10.178

Authors

Kenyon Johanna J. ¹ , Kasimova Anastasiya A. ² , Sviridova Anastasiya N. ³ , Shpirt Anna M. ² , Shneider Mikhail M. ⁴ , Mikhaylova Yuliya V. ⁵ , Shelenkov Andrei A. ⁵ , Popova Anastasiya V. ⁶ , Perepelov Andrei V. ² , Shashkov Alexander S. ² , Dmitrenok Andrei S. ² , Chizov Alexander O. ² , Knirel Yuriy A. ²

Affiliations

1	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology. Brisbane, Australia
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Science, Moscow, Russia
3	D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia
4	M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia
5	Central Scientific Research Institute of Epidemiology, Moscow, Russia.
6	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow, Region, Russia

Whole genome sequence from Acinetobacter baumannii isolate Ab-46-1632 reveals a novel KL144 capsular polysaccharide (CPS) biosynthesis gene cluster, which carries genes for d-glucuronic acid (D-GlcA) and l-rhamnose (l-Rha) synthesis. The CPS was extracted from Ab-46-1632 and studied by 1H and 13C NMR spectroscopy, including a two-dimensional 1H,13C HMBC experiment and Smith degradation. The CPS was found to have a hexasaccharide repeat unit composed of four l-Rhap residues and one residue each of d-GlcpA and N-acetyl-d-glucosamine (D-GlcpNAc) consistent with sugar synthesis genes present in KL144. The K144 CPS structure was established and found to be related to those of A. baumannii K55, K74, K85, and K86. A comparison of the corresponding gene clusters to KL144 revealed a number of shared glycosyltransferase genes correlating to shared glycosidic linkages in the structures. One from the enzymes, encoded by only KL144 and KL86, is proposed to be a novel multifunctional rhamnosyltransfaerase likely responsible for synthesis of a shared α-l-Rhap-(1 → 2)-α-L-Rhap-(1 → 3)-L-Rhap trisaccharide fragment in the K144 and K86 structures.

Kenyon J.J. , Kasimova A.A. , Sviridova A.N. , Shpirt A.M. , Shneider M.M. , Mikhaylova Y.V. , Shelenkov A.A. , Popova A.V. , Perepelov A.V. , Shashkov A.S. , Dmitrenok A.S. , Chizov A.O. , Knirel Y.A.
Correlation of Acinetobacter baumannii K144 and K86 capsular polysaccharide structures with genes at the K locus reveals the involvement of a novel multifunctional rhamnosyltransferase for structural synthesis
International Journal of Biological Macromolecules. 2021. V.193. P.1294-1300. DOI: 10.1016/j.ijbiomac.2021.10.178 WOS Scopus OpenAlex

Web of science:	WOS:000734363900003
Scopus:	2-s2.0-85118967854
OpenAlex:	W3209268262

DB	Citing
OpenAlex	12
Scopus	10
Web of science	10