Structure of the K87 capsular polysaccharide and KL87 gene cluster of Acinetobacter baumannii LUH5547 reveals a heptasaccharide repeating unit

Structure of the K87 capsular polysaccharide and KL87 gene cluster of Acinetobacter baumannii LUH5547 reveals a heptasaccharide repeating unit Научная публикация

Журнал

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Вых. Данные

Год: 2021, Том: 509, Номер статьи : 108439, Страниц : DOI: 10.1016/j.carres.2021.108439

Авторы

Arbatsky Nikolay P. ¹ , Popova Anastasiya V. ^2,3 , Shneider Mikhail M. ⁴ , Shashkov Alexander S. ¹ , Hall Ruth M. ⁵ , Kenyon Johanna J. ⁶ , Knirel Yuriy A. ¹

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia;
2	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
3	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
4	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
5	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
6	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology. Brisbane, Australia

K87 capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii isolate LUH5547 that carries the KL87 capsule biosynthesis gene cluster at the chromosomal K locus. Studies by sugar analysis, selective chemical cleavages, and 1D and 2D 1H and 13C NMR spectroscopy showed that the CPS has a branched heptasaccharide repeat (K unit) containing one residue each of d-glucose (d-Glсp), d-glucuronic acid (d-GlсpA), N-acetyl-d-glucosamine (d-GlсpNAc), 6-deoxy-l-talose (l-6dTalp), and three residues of l-rhamnose (l-Rhap). The following structure of the CPS was established: →3) − α − L − Rhap − (1→2) − α − L − Rhap − (1→3) − α − L − 6dTalp − (1→3) − β − D − GlcpNAc − (1→ 2 ↑ 1 β − D − GlcpA − (4←1) − α − D − Glcp (2←1) − α − L − Rhap The position of a minor O-acetyl group present in the CPS was not determined. Functions of enzymes coded by genes in the KL87 gene cluster were tentatively assigned and found to be consistent with the CPS structure.

Arbatsky N.P. , Popova A.V. , Shneider M.M. , Shashkov A.S. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
Structure of the K87 capsular polysaccharide and KL87 gene cluster of Acinetobacter baumannii LUH5547 reveals a heptasaccharide repeating unit
Carbohydrate Research. 2021. V.509. 108439 . DOI: 10.1016/j.carres.2021.108439 WOS Scopus OpenAlex

Web of science:	WOS:000701954000003
Scopus:	2-s2.0-85115137728
OpenAlex:	W3197085163

БД	Цитирований
OpenAlex	13
Scopus	10
Web of science	10