Glycosaminoglycans from the Starfish Lethasterias fusca: Structures and Influence on Hematopoiesis

Glycosaminoglycans from the Starfish Lethasterias fusca: Structures and Influence on Hematopoiesis Full article

Journal

Marine Drugs
ISSN: 1660-3397

Output data

Year: 2023, Volume: 21, Number: 4, Article number : 205, Pages count : DOI: 10.3390/md21040205

Authors

Bilan Maria I. ¹ , Anisimova Natalia Yu. ² , Tokatly Alexandra I. ¹ , Nikogosova Sofya P. ¹ , Vinnitskiy Dmitriy Z. ¹ , Ustyuzhanina Nadezhda E. ¹ , Dmitrenok Andrey S. ¹ , Tsvetkova Evgenia A. ¹ , Kiselevskiy Mikhail V. ² , Nifantiev Nikolay E. ¹ , Usov Anatolii I. ¹

Affiliations

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia
2	FSBI N.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, Moscow 115458, Russia

Crude anionic polysaccharides extracted from the Pacific starfish Lethasterias fusca were purified by anion-exchange chromatography. The main fraction LF, having MW 14.5 kDa and dispersity 1.28 (data of gel-permeation chromatography), was solvolytically desulfated and giving rise to preparation LF-deS with a structure of dermatan core [→3)-β-d-GalNAc-(1→4)-α-l-IdoA-(1→]n, which was identified according to NMR spectroscopy data. Analysis of the NMR spectra of the parent fraction LF led to identification of the main component as dermatan sulfate LF-Derm →3)-β-d-GalNAc4R-(1→4)-α-l-IdoA2R3S-(1→ (where R was SO3 or H), bearing sulfate groups at O-3 or both at O-2 and O-3 of α-l-iduronic acid, as well as at O-4 of some N-acetyl-d-galactosamine residues. The minor signals in NMR spectra of LF were assigned as resonances of heparinoid LF-Hep composed of the fragments →4)-α-d-GlcNS3S6S-(1→4)-α-l-IdoA2S3S-(1→. The 3-O-sulfated and 2,3-di-O-sulfated iduronic acid residues are very unusual for natural glycosaminoglycans, and further studies are needed to elucidate their possible specific influence on the biological activity of the corresponding polysaccharides. To confirm the presence of these units in LF-Derm and LF-Hep, a series of variously sulfated model 3-aminopropyl iduronosides were synthesized and their NMR spectra were compared with those of the polysaccharides. Preparations LF and LF-deS were studied as stimulators of hematopoiesis in vitro. Surprisingly, it was found that both preparations were active in these tests, and hence, the high level of sulfation is not necessary for hematopoiesis stimulation in this particular case.

Bilan M.I. , Anisimova N.Y. , Tokatly A.I. , Nikogosova S.P. , Vinnitskiy D.Z. , Ustyuzhanina N.E. , Dmitrenok A.S. , Tsvetkova E.A. , Kiselevskiy M.V. , Nifantiev N.E. , Usov A.I.
Glycosaminoglycans from the Starfish Lethasterias fusca: Structures and Influence on Hematopoiesis
Marine Drugs. 2023. V.21. N4. 205 . DOI: 10.3390/md21040205 WOS Scopus OpenAlex

≡ Web of science:	WOS:000977362100001
≡ Scopus:	2-s2.0-85153755510
≡ OpenAlex:	W4361004914