Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2020, Volume: 21, Number: 9, Article number : 3170, Pages count : DOI: 10.3390/ijms21093170

Authors

Shneider Mikhail M ¹ , Lukianova Anna A ^2,1 , Evseev Peter V ¹ , Shpirt Anna M ³ , Kabilov Marsel R ⁴ , Tokmakova Anna D ^1,5 , Miroshnikov Kirill K ^1,6 , Obraztsova Ekaterina A ¹ , Baturina Olga A ⁴ , Shashkov Alexander S ³ , Ignatov Alexander N ⁷ , Knirel Yuriy A ³ , Miroshnikov Konstantin A ¹

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
2	Department of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
3	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
4	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia
5	Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, 109377 Moscow, Russia
6	Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of biotechnology”, Russian Academy of Sciences, 117312 Moscow, Russia
7	Research Center “PhytoEngineering” Ltd., Rogachevo, 141880 Moscow, Russia

Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

Shneider M.M. , Lukianova A.A. , Evseev P.V. , Shpirt A.M. , Kabilov M.R. , Tokmakova A.D. , Miroshnikov K.K. , Obraztsova E.A. , Baturina O.A. , Shashkov A.S. , Ignatov A.N. , Knirel Y.A. , Miroshnikov K.A.
Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide
International Journal of Molecular Sciences. 2020. V.21. N9. 3170 . DOI: 10.3390/ijms21093170 WOS Scopus OpenAlex

Web of science:	WOS:000535581700141
Scopus:	2-s2.0-85084276761
OpenAlex:	W3022658369

DB	Citing
OpenAlex	15
Scopus	13
Web of science	10