Novel Acinetobacter baumannii Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy

Novel Acinetobacter baumannii Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy Научная публикация

Журнал

Viruses-Basel
ISSN: 1999-4915

Вых. Данные

Год: 2021, Том: 13, Номер: 6, Номер статьи : 978, Страниц : DOI: 10.3390/v13060978

Авторы

Shchurova Anastasia S ^1,2 , Shneider Mikhail M ³ , Arbatsky Nikolay P ⁴ , Shashkov Alexander S ⁴ , Chizhov Alexander O ⁴ , Skryabin Yuriy P ² , Mikhaylova Yulia V ⁵ , Sokolova Olga S ^6,7 , Shelenkov Andrey A ⁵ , Miroshnikov Konstantin A ³ , Knirel Yuriy A ⁴ , Popova Anastasia V ²

Организации

1	Moscow Institute of Physics and Technology (National Research University), Institutskiy per. 9, Dolgoprudny, 141700 Moscow Region, Russia
2	State Research Center for Applied Microbiology and Biotechnology, 24 “Quarter A” Territory, Obolensk, City District Serpukhov, 142279 Moscow Region, Russia
3	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
4	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
5	Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia
6	Biology Department, Lomonosov Moscow State University, Leninskie Gory 1, 119234 Moscow, Russia
7	Biology Department, Shenzhen MSU-BIT University, Ruyi Rd. 299, Longgang District, Shenzhen 518172, China

Acinetobacter baumannii, one of the most significant nosocomial pathogens, is capable of producing structurally diverse capsular polysaccharides (CPSs) which are the primary receptors for A. baumannii bacteriophages encoding polysaccharide-degrading enzymes. To date, bacterial viruses specifically infecting A. baumannii strains belonging to more than ten various capsular types (K types) were isolated and characterized. In the present study, we investigate the biological properties, genomic organization, and virus–bacterial host interaction strategy of novel myovirus TaPaz isolated on the bacterial lawn of A. baumannii strain with a K47 capsular polysaccharide structure. The phage linear double-stranded DNA genome of 93,703 bp contains 178 open reading frames. Genes encoding two different tailspike depolymerases (TSDs) were identified in the phage genome. Recombinant TSDs were purified and tested against the collection of A. baumannii strains belonging to 56 different K types. One of the TSDs was demonstrated to be a specific glycosidase that cleaves the K47 CPS by the hydrolytic mechanism.

Shchurova A.S. , Shneider M.M. , Arbatsky N.P. , Shashkov A.S. , Chizhov A.O. , Skryabin Y.P. , Mikhaylova Y.V. , Sokolova O.S. , Shelenkov A.A. , Miroshnikov K.A. , Knirel Y.A. , Popova A.V.
Novel Acinetobacter baumannii Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy
Viruses-Basel. 2021. V.13. N6. 978 . DOI: 10.3390/v13060978 WOS Scopus OpenAlex

Web of science:	WOS:000666715800001
Scopus:	2-s2.0-85107376804
OpenAlex:	W3164025651

БД	Цитирований
OpenAlex	20
Web of science	19
Scopus	19