Fucosylated Chondroitin Sulfates with Rare Disaccharide Branches from the Sea Cucumbers Psolus peronii and Holothuria nobilis: Structures and Influence on Hematopoiesis

Fucosylated Chondroitin Sulfates with Rare Disaccharide Branches from the Sea Cucumbers Psolus peronii and Holothuria nobilis: Structures and Influence on Hematopoiesis Научная публикация

Журнал

Pharmaceuticals
ISSN: 1424-8247

Вых. Данные

Год: 2023, Том: 16, Номер: 12, Номер статьи : 1673, Страниц : DOI: 10.3390/ph16121673

Авторы

Ustyuzhanina Nadezhda E. ¹ , Bilan Maria I. ¹ , Anisimova Natalia Yu. ² , Nikogosova Sofya P. ¹ , Dmitrenok Andrey S. ¹ , Tsvetkova Evgenia A. ¹ , Panina Elena G. ³ , Sanamyan Nadezhda P. ³ , Avilov Sergey A. ⁴ , Stonik Valentin A. ⁴ , Kiselevskiy Mikhail V. ² , Usov Anatolii I. ¹ , Nifantiev Nikolay E. ¹

Организации

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia
2	FSBI N.E.N. Blokhin National Medical Research Center of Oncology, Kashirskoye sh. 24, Moscow 115458, Russia
3	Kamchatka Branch of Pacific Geographical Institute FEB RAS, Petropavlovsk-Kamchatsky 683000, Russia
4	G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia

Two fucosylated chondroitin sulfates were isolated from the sea cucumbers Psolus peronii and Holothuria nobilis using a conventional extraction procedure in the presence of papain, followed by anion-exchange chromatography on DEAE-Sephacel. Their composition was characterized in terms of quantitative monosaccharide and sulfate content, and structures were mainly elucidated using 1D- and 2D-NMR spectroscopy. As revealed by the data of the NMR spectra, both polysaccharides along with the usual fucosyl branches contained rare disaccharide branches α-D-GalNAc4S6R-(1→2)-α-L-Fuc3S4R → attached to O-3 of the GlcA of the backbone (R = H or SO3−). The polysaccharides were studied as stimulators of hematopoiesis in vitro using mice bone marrow cells as the model. The studied polysaccharides were shown to be able to directly stimulate the proliferation of various progenitors of myelocytes and megakaryocytes as well as lymphocytes and mesenchymal cells in vitro. Therefore, the new fucosylated chondroitin sulfates can be regarded as prototype structures for the further design of GMP-compatible synthetic analogs for the development of new-generation hematopoiesis stimulators.

Ustyuzhanina N.E. , Bilan M.I. , Anisimova N.Y. , Nikogosova S.P. , Dmitrenok A.S. , Tsvetkova E.A. , Panina E.G. , Sanamyan N.P. , Avilov S.A. , Stonik V.A. , Kiselevskiy M.V. , Usov A.I. , Nifantiev N.E.
Fucosylated Chondroitin Sulfates with Rare Disaccharide Branches from the Sea Cucumbers Psolus peronii and Holothuria nobilis: Structures and Influence on Hematopoiesis
Pharmaceuticals. 2023. V.16. N12. 1673 . DOI: 10.3390/ph16121673 WOS Scopus OpenAlex

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