Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide

Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2024, Volume: 535, Article number : 109020, Pages count : DOI: 10.1016/j.carres.2023.109020

Authors

Arbatsky Nikolay P. ¹ , Kasimova Anastasiya A. ¹ , Shashkov Alexander S. ¹ , Shneider Mikhail M. ² , Popova Anastasiya V. ^3,4 , Perepelov Andrey V. ¹ , Hall Ruth M. ⁵ , Kenyon Johanna J. ^6,7 , Knirel Yuriy A. ¹

Affiliations

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997, Moscow, Russia
3	Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
4	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
5	School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia
6	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
7	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast, Australia

K63 capsular polysaccharide produced by Acinetobacter baumannii isolate LUH5551 (previously designated isolate O24) was re-examined using sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. Though previously reported as O24 consisting of linear tetrasaccharide units that include a 7-acetamido-5-acylamino form of 8-epilegionaminic acid [8eLeg5R7Ac, acylated at C5 with (S)-3-hydroxybutanoyl or acetyl (1:1)], the elucidated structure of the K63 type capsule was found to include a derivative of 5,7-diamino-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulosonic (legionaminic) acid, Leg5Ac7R, where R is either (S)-3-hydroxybutanoyl or an acetyl group (∼1:1 ratio). This finding is consistent with the presence of the lgaABCHIFG gene module for Leg5Ac7R biosynthesis in the KL63 gene cluster at the capsular polysaccharide (CPS) biosynthesis K locus in the LUH5551 genome. The glycosyltransferases (Gtrs) and Wzy polymerase encoded by KL63 were assigned to linkages in the linear K63 tetrasaccharide unit and linkage of the K63 units.

Arbatsky N.P. , Kasimova A.A. , Shashkov A.S. , Shneider M.M. , Popova A.V. , Perepelov A.V. , Hall R.M. , Kenyon J.J. , Knirel Y.A.
Revised structure of the polysaccharide from Acinetobacter baumannii LUH5551 assigned as the K63 type capsular polysaccharide
Carbohydrate Research. 2024. V.535. 109020 . DOI: 10.1016/j.carres.2023.109020 WOS Scopus OpenAlex

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