Реферат: The O-specific polysaccharide of H. alvei strain PCM 1222 has a branched hexasaccharide repeating unit containing d-galactose, l-rhamnose, d-ribose, d-galacturonic acid, and 2-acetamido-2-deoxy-d-glucose in the ratios 1:2:1:1:1, as well as 2-aminoethyl phosphate (EtNP) and O-acetyl groups in nonstoichiometric amounts. The polysaccharide was modified by carboxyl reduction, O-deacetylation, and dephosphorylation with 48% hydrofluoric acid, the last reaction being accompanied by removal of the lateral residue of β-galactofuranose. The modified polysaccharides were studied by methylation analysis and 1H and 13C NMR spectroscopy, including 2D correlation spectroscopy (COSY), H-detected 1H, 13C and 1H, 31P heteronuclear multiple-quantum coherence (HMQC), 1D NOE, 2D rotating-frame NOE spectroscopy (ROESY), and 2D combined total correlation spectroscopy (TOCSY) and ROESY (TORO). The following structure of the O-deacetylated polysaccharide was established. In different batches of the polysaccharide, the content of EtNP varied from 0.35 to 0.55 and that of the O-acetyl groups from 0.05 to 0.4 per repeating unit. It was tentatively suggested that the O-acetyl group is located at position 4 of a rhamnosyl residue.

Библиографическая ссылка: Toukach F.V. , Shashkov A.S. , Katzenellenbogen E. , Kocharova N.A. , Czarny A. , Knirel Y.A. , Romanowska E. , Kochetkov N.K.
Structure of the O-specific polysaccharide of Hafnia alvei PCM 1222 containing 2-aminoethyl phosphate
Carbohydrate Research. 1996. V.295. P.117-126. DOI: 10.1016/s0008-6215(96)90130-5 WOS Scopus OpenAlex

Идентификаторы БД:

≡ Web of science:	WOS:A1996VZ16900010
≡ Scopus:	2-s2.0-0029952936
≡ OpenAlex:	W1971110688

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