Structural and immunochemical studies on the lipopolysaccharide of the ‘T-antigen’-containing mutantProteus mirabilisR14/ 1959

Structural and immunochemical studies on the lipopolysaccharide of the ‘T-antigen’-containing mutantProteus mirabilisR14/ 1959 Научная публикация

Журнал

Fems Immunology and Medical Microbiology
ISSN: 0928-8244 , E-ISSN: 1574-695X

Вых. Данные

Год: 1996, Том: 13, Номер: 2, Страницы: 113-121 Страниц : 9 DOI: 10.1111/j.1574-695x.1996.tb00224.x

Авторы

Bartodziejska Beata ¹ , Radziejewska-Lebrecht Joanna ¹ , Lipinska Maria ¹ , Knirel Yuriy A. ² , Kononov Leonid O. ² , Chernyak Anatoly Y. ² , Mayer Hubert ³ , Rozalski Antoni ¹

Организации

1	Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, PL 90-237 Lodz, Poland
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russian Federation
3	Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg i. Br., Germany

In DOC-PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T-antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained D-glucose, D-galacturonic acid (D-GalA), and D-GlcNAc in molar ratios 2:1:1 and was structurally different from the O-antigen of the parental S-strain P. mirahilis Sl959 but identical to the O-antigen of another S-strain Proteus penneri 42. The importance of a D-GalA(L-Lys)-containing epitope, most likely present in the core region of LPS, and of GalA present in the T-antigen chain in manifesting the serological specificity of P. mirabilis Rl4/ 1959 were revealed using rabbit polyclonal homologouas nd heterologous R- and O-specific antisera and the appropriate antigens. including synthetic antigens which represent partial structures of various Proteus LPS.

Bartodziejska B. , Radziejewska-Lebrecht J. , Lipinska M. , Knirel Y.A. , Kononov L.O. , Chernyak A.Y. , Mayer H. , Rozalski A.
Structural and immunochemical studies on the lipopolysaccharide of the ‘T-antigen’-containing mutantProteus mirabilisR14/ 1959
Fems Immunology and Medical Microbiology. 1996. V.13. N2. P.113-121. DOI: 10.1111/j.1574-695x.1996.tb00224.x OpenAlex

OpenAlex:

W1985917765

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