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Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina Full article

Journal International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130
Output data Year: 2021, Volume: 182, Pages: 2019-2023 Pages count : 5 DOI: 10.1016/j.ijbiomac.2021.05.187
Authors Zdorovenko Evelina L. 1 , Dmitrenok Andrey S. 1 , Masi Marco 2 , Castaldi Stefany 3 , Muzio Federico M. 4 , Isticato Rachele 3 , Valverde Claudio 4 , Knirel Yuriy A. 1
Affiliations
1 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia.
2 Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via cintia 4, 80126, Naples, Italy.
3 Department of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.
4 Laboratorio de Fisiología y Genética de Bacterias Beneficiosas para Plantas-Centro de Bioquímica y Microbiología del Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes-CONICET, Buenos Aires, Argentina.

Abstract: An O-specific polysaccharide (OPS) was isolated from the lipopolysaccharide (LPS) of Pseudomonas donghuensis SVBP6, a bacterium with a broad-spectrum antifungal activity in vitro, particularly that against Macrophomina phaseolina. This latter is one of the most virulent and dangerous pathogens of plants, including soybean which is an economically important crop in Argentina today. The OPS was studied by sugar analysis and spectroscopy (1D and 2D 1H and 13C NMR) showing the following trisaccharide repeating unit: →6)-ɑ-D-ManpNAc-(1 → 3)-β-l-Rhap-(1 → 4)-β-D-Glcp-(1→. The crude LPS, the purified LPS and the O-chain were assayed for their antifungal activity against M. phaseolina at 25, 50, 100, and 200 μg plug−1. The results showed that the crude LPS best inhibition was at 200 μg plug−1, able to inhibit the fungus growth by about 45%, while purified LPS and the corresponding OPS, in the same condition, reduced fungus growth by 65%, and 75%, respectively. Furthermore, the purified LPS and OPS significantly reduced the growth of M. phaseolina already at 100 μg plug−1 compared to the crude LPS. The structure of the O-chain is unique among the bacterial LPS and this is the first time that both the antifungal activity of a bacterial LPS and its corresponding O-chain were described.
Cite: Zdorovenko E.L. , Dmitrenok A.S. , Masi M. , Castaldi S. , Muzio F.M. , Isticato R. , Valverde C. , Knirel Y.A.
Structural studies on the O-specific polysaccharide of the lipopolysaccharide from Pseudomonas donghuensis strain SVBP6, with antifungal activity against the phytopathogenic fungus Macrophomina phaseolina
International Journal of Biological Macromolecules. 2021. V.182. P.2019-2023. DOI: 10.1016/j.ijbiomac.2021.05.187 WOS Scopus OpenAlex
Identifiers:
Web of science: WOS:000669451600004
Scopus: 2-s2.0-85108116326
OpenAlex: W3165657274
Citing:
DB Citing
OpenAlex 10
Scopus 8
Web of science 8
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