Digital biology approach for macroscale studies of biofilm growth and biocide effects with electron microscopy

Digital biology approach for macroscale studies of biofilm growth and biocide effects with electron microscopy Full article

Journal

Digital Discovery
ISSN: 2635-098X

Output data

Year: 2023, Volume: 2, Number: 5, Pages: 1522-1539 Pages count : 18 DOI: 10.1039/d3dd00048f

Authors

Kozlov Konstantin S. ^1,2 , Boiko Daniil A. ² , Detusheva Elena V. ³ , Detushev Konstantin V. ³ , Pentsak Evgeniy O. ² , Vereshchagin Anatoly N. ² , Ananikov Valentine P. ^1,2

Affiliations

1	Lomonosov Moscow State University, Chemistry Department, Leninskie Gory 1/3, Moscow, 119991, Russia
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow, 119991, Russia
3	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia

Microbial interactions are one of the major topics of current research due to their great societal relevance. It is now established that biofilms—associations of microorganisms, exchanging various chemical compounds, including proteins and nucleic acids—are capable of promoting horizontal transfer of resistance genes. However, our understanding of the processes occurring in biofilms is rather limited. A possible method to partly overcome this problem is the implementation of highly efficient imaging and mapping of these structures. This work proposes a combination of automated scanning electron microscopy (SEM) and a comprehensive software system that uses deep neural networks to perform an in-depth analysis of biofilms. Time-dependent, high-throughput mapping of biofilm electron microscopy images was achieved using deep learning and allowed microscale data analysis of visible to the eye biofilm-covered area (i.e., at the macroscale). For this study, to the best of our knowledge, the first matrix and cell-annotated biofilm segmentation dataset was prepared. We show that the presented approach can be used to process statistical data investigation of biofilm samples in a volume, where automation is essential (>70 000 separate bacterial cells studied; >1000 times faster than regular manual analysis). To evaluate the approach, multiple time steps of biofilm development were analyzed by first-to-date kinetic modeling of biofilms with SEM, revealing the complex dynamics of biofilm formation. Moreover, it was shown that the described procedure is capable of capturing differences between antibiotics and antimicrobial compounds applied to studied biofilms.

Kozlov K.S. , Boiko D.A. , Detusheva E.V. , Detushev K.V. , Pentsak E.O. , Vereshchagin A.N. , Ananikov V.P.
Digital biology approach for macroscale studies of biofilm growth and biocide effects with electron microscopy
Digital Discovery. 2023. V.2. N5. P.1522-1539. DOI: 10.1039/d3dd00048f OpenAlex

OpenAlex:

W4386711835

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