Depolymerisation of the Klebsiella pneumoniae Capsular Polysaccharide K21 by Klebsiella Phage K5

Depolymerisation of the Klebsiella pneumoniae Capsular Polysaccharide K21 by Klebsiella Phage K5 Full article

Journal

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Output data

Year: 2023, Volume: 24, Number: 24, Article number : 17288, Pages count : DOI: 10.3390/ijms242417288

Authors

Lukianova Anna A. ¹ , Shneider Mikhail M. ¹ , Evseev Peter V. ¹ , Egorov Mikhail V. ¹ , Kasimova Anastasiya A. ² , Shpirt Anna M. ² , Shashkov Alexander S. ² , Knirel Yuriy A. ² , Kostryukova Elena S. ³ , Miroshnikov Konstantin A. ¹

Affiliations

1	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, 117997 Moscow, Russia
2	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prosp. 47, 119991 Moscow, Russia
3	Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Malaya Pirogovskaya Str. 1, 119435 Moscow, Russia

Klebsiella pneumoniae is a pathogen associated with various infection types, which often exhibits multiple antibiotic resistance. Phages, or bacterial viruses, have an ability to specifically target and destroy K. pneumoniae, offering a potential means of combatting multidrug-resistant infections. Phage enzymes are another promising therapeutic agent that can break down bacterial capsular polysaccharide, which shields K. pneumoniae from the immune response and external factors. In this study, Klebsiella phage K5 was isolated; this phage is active against Klebsiella pneumoniae with the capsular type K21. It was demonstrated that the phage can effectively lyse the host culture. The adsorption apparatus of the phage has revealed two receptor-binding proteins (RBPs) with predicted polysaccharide depolymerising activity. A recombinant form of both RBPs was obtained and experiments showed that one of them depolymerised the capsular polysaccharide K21. The structure of this polysaccharide and its degradation fragments were analysed. The second receptor-binding protein showed no activity on capsular polysaccharide of any of the 31 capsule types tested, so the substrate for this enzyme remains to be determined in the future. Klebsiella phage K5 may be considered a useful agent against Klebsiella infections.

Lukianova A.A. , Shneider M.M. , Evseev P.V. , Egorov M.V. , Kasimova A.A. , Shpirt A.M. , Shashkov A.S. , Knirel Y.A. , Kostryukova E.S. , Miroshnikov K.A.
Depolymerisation of the Klebsiella pneumoniae Capsular Polysaccharide K21 by Klebsiella Phage K5
International Journal of Molecular Sciences. 2023. V.24. N24. 17288 . DOI: 10.3390/ijms242417288 WOS Scopus OpenAlex

Web of science:	WOS:001132370000001
Scopus:	2-s2.0-85180712470
OpenAlex:	W4389566955

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OpenAlex	5
Scopus	1
Web of science	5