Microbiological Evaluation of Novel Bis-Quaternary Ammonium Compounds: Clinical Strains, Biofilms, and Resistance Study

Microbiological Evaluation of Novel Bis-Quaternary Ammonium Compounds: Clinical Strains, Biofilms, and Resistance Study Full article

Journal

Pharmaceuticals
ISSN: 1424-8247

Output data

Year: 2022, Volume: 15, Number: 5, Article number : 514, Pages count : DOI: 10.3390/ph15050514

Authors

Frolov Nikita ¹ , Detusheva Elena ² , Fursova Nadezhda ² , Ostashevskaya Irina ^3,1 , Vereshchagin Anatoly ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
2	State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279 Serpukhov, Moscow Region, Russia
3	Faculty of Chemistry, Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia

This work is devoted to the investigation of biocidal properties of quaternary ammonium compounds (QACs) based on pyridine structures with aromatic spacers, and their widely known analogs, against clinically significant microorganisms. This study is focused on investigating their antimicrobial activity (minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs)), antibiofilm properties (minimum biofilm inhibitory concentrations (MBICs) and minimum biofilm eradication concentrations (MBECs)), synergetic effect with different alcohols in antiseptic formulations, and bacterial resistance development. It was shown that all combined analogue preparations had a higher level of antibacterial activity against the tested bacterial strains, with a 16- to 32-fold reduction in MICs and MBCs compared to previously used antiseptic preparations. Moreover, hit-QACs demonstrated a stable effect against Gram-negative E. coli, K. pneumoniae, and A. baumannii within a month of incubation. Overall results indicated a high level of antibacterial activity of pyridine-based QACs.

Frolov N. , Detusheva E. , Fursova N. , Ostashevskaya I. , Vereshchagin A.
Microbiological Evaluation of Novel Bis-Quaternary Ammonium Compounds: Clinical Strains, Biofilms, and Resistance Study
Pharmaceuticals. 2022. V.15. N5. 514 . DOI: 10.3390/ph15050514 WOS Scopus OpenAlex

Web of science:	WOS:000803451300001
Scopus:	2-s2.0-85129762058
OpenAlex:	W4224313805

DB	Citing
OpenAlex	22
Scopus	21
Web of science	21