Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan

Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan Full article

Journal

Journal of Fungi
ISSN: 2309-608X

Output data

Year: 2021, Volume: 7, Number: 7, Article number : 504, Pages count : DOI: 10.3390/jof7070504

Authors

Krylov Vadim B. ¹ , Solovev Arsenii S. ¹ , Puchkin Ilya A. ¹ , Yashunsky Dmitry V. ¹ , Antonets Anna V. ^1,2 , Kutsevalova Olga Y. ³ , Nifantiev Nikolay E. ¹

Affiliations

1	Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia.
2	Medical Genetic Center, Rostov-on-Don State Medical University, Nakhichevansky, 29, 344022 Rostov-on-Don, Russia
3	National Medical Research Center of Oncology, Laboratory of Clinical Microbiology, 14 Liniya Str., 63, 344037 Rostov-on-Don, Russia

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

Krylov V.B. , Solovev A.S. , Puchkin I.A. , Yashunsky D.V. , Antonets A.V. , Kutsevalova O.Y. , Nifantiev N.E.
Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan
Journal of Fungi. 2021. V.7. N7. 504 . DOI: 10.3390/jof7070504 WOS Scopus OpenAlex

≡ Web of science:	WOS:000676154800001
≡ Scopus:	2-s2.0-85122909634
≡ OpenAlex:	W3174253113