Mechanisms of Acinetobacter baumannii Capsular Polysaccharide Cleavage by Phage Depolymerases

Mechanisms of Acinetobacter baumannii Capsular Polysaccharide Cleavage by Phage Depolymerases Full article

Journal

Biokhimiya
ISSN: 0006-2979 , E-ISSN: 1608-3040

Output data

Year: 2020, Volume: 85, Number: 5, Pages: 567-574 Pages count : 8 DOI: 10.1134/s0006297920050053

Authors

Knirel Y.A. ¹ , Shneider M.M. ^2,3 , Popova A.V. ^2,4,5 , Kasimova A.A. ^6,1 , Senchenkova S.N. ¹ , Shashkov A.S. ¹ , Chizhov A.O. ¹

Affiliations

1	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia
3	Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia
4	Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia
6	Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Aerobic gram-negative bacterium Acinetobacter baumannii has recently become one of the most relevant pathogens associated with hospital-acquired infections worldwide. A. baumannii produces a capsule around the cell, which represents a thick viscous layer of structurally variable capsular polysaccharide (CPS). The capsule protects the bacteria against unfavorable environmental factors and biological systems, including bacteriophages and host immune system. Many A. baumannii phages have structural depolymerases (tailspikes) that specifically recognize and digest bacterial CPS. In this work, we studied the interaction of tailspike proteins of four lytic depolymerase-carrying phages with A. baumannii CPS. Depolymerases of three bacteriophages (Fri1, AS12, and BS46) were identified as specific glycosidases that cleave the CPS of A. baumannii strains 28, 1432, and B05, respectively, by the hydrolytic mechanism. The gp54 depolymerase from bacteriophage AP22 was characterized as a polysaccharide lyase that cleaves the CPS of A. baumannii strain 1053 by β-elimination at hexuronic acid (ManNAcA) residues.

Knirel Y.A. , Shneider M.M. , Popova A.V. , Kasimova A.A. , Senchenkova S.N. , Shashkov A.S. , Chizhov A.O.
Mechanisms of Acinetobacter baumannii Capsular Polysaccharide Cleavage by Phage Depolymerases
Biokhimiya. 2020. V.85. N5. P.567-574. DOI: 10.1134/s0006297920050053 WOS Scopus OpenAlex

Web of science:	WOS:000534300500004
Scopus:	2-s2.0-85085034177
OpenAlex:	W3028074954

DB	Citing
OpenAlex	35
Scopus	31
Web of science	31