Fluorescence Polarization Assay for Infection Diagnostics: A Review | Sciact

Fluorescence Polarization Assay for Infection Diagnostics: A Review Обзор

Журнал

Molecules
ISSN: 1420-3049

Вых. Данные

Год: 2024, Том: 29, Номер: 19, Номер статьи : 4712, Страниц : DOI: 10.3390/molecules29194712

Авторы

Eremin Sergei A. ¹ , Mukhametova Liliya I. ¹ , Krylov Vadim B. ² , Nifantiev Nikolay E. ²

Организации

1	Chemical Department, M.V. Lomonosov Moscow State University, Leninsky Gory, 1, 119991 Moscow, Russia
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, 119991 Moscow, Russia

Rapid and specific diagnosis is necessary for both the treatment and prevention of infectious diseases. Bacteria and viruses that enter the bloodstream can trigger a strong immune response in infected animals and humans. The fluorescence polarization assay (FPA) is a rapid and accurate method for detecting specific antibodies in the blood that are produced in response to infection. One of the first examples of FPA is the non-competitive test for detecting brucellosis in animals, which was followed by the development of other protocols for detecting various infections. Fluorescently labeled polysaccharides (in the case of brucellosis and salmonellosis) or specific peptides (in the case of tuberculosis and salmonellosis, etc.) can be used as biorecognition elements for detecting infections. The availability of new laboratory equipment and mobile devices for fluorescence polarization measurements outside the laboratory has stimulated the development of new fluorescence polarization assays (FPAs) and the emergence of commercial kits on the market for the detection of brucellosis, tuberculosis, and equine infectious anemia viruses. It has been shown that, in addition to antibodies, the FPA method can detect both viruses and nucleic acids. The development of more specific and sensitive biomarkers is essential for the diagnosis of infections and therapy monitoring. This review summarizes studies published between 2003 and 2023 that focus on the detection of infections using FPA. Furthermore, it demonstrates the potential for using new biorecognition elements (e.g., aptamers, proteins, peptides) and the combined use of FPA with new technologies, such as PCR and CRISPR/Cas12a systems, for detecting various infectious agents.

Eremin S.A. , Mukhametova L.I. , Krylov V.B. , Nifantiev N.E.
Fluorescence Polarization Assay for Infection Diagnostics: A Review
Molecules. 2024. V.29. N19. 4712 . DOI: 10.3390/molecules29194712 WOS Scopus OpenAlex

≡ Web of science:	WOS:001332619200001
≡ Scopus:	2-s2.0-85206541958
≡ OpenAlex:	W4403194314