The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15-4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology

The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15-4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2024, Volume: 545, Article number : 109273, Pages count : DOI: 10.1016/j.carres.2024.109273

Authors

Arbatsky Nikolay P ¹ , Shashkov Alexander S ¹ , Shneider Mikhail M ² , Mikhailova Yulia V ³ , Shelenkov Andrey A ³ , Sheck Eugene A ⁴ , Kasimova Anastasia A ¹ , Kalinchuk Nadezhda A ¹ , Kenyon Johanna J ^5,6 , Knirel Yuriy A ¹

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
2	M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
3	Central Research Institute for Epidemiology, Moscow, Russia.
4	Institute of Antimicrobial Chemotherapy (IAC), Smolensk State Medical University, Smolensk, Russia.
5	School of Pharmacy and Medical Sciences, Health Group, Griffith University, Gold Coast Campus, Southport, Australia
6	Institute for Biomedicine and Glycomics, Griffith University, Gold Coast Campus, Southport, Australia

Capsular polysaccharide (CPS) is a heteroglycan that coats the cell surface of most isolates of the important Gram-negative bacterial pathogen, Acinetobacter baumannii. Strain MAR 15–4076, a clinical isolate recovered in Russia in 2015, was found to carry the KL129 sequence at the CPS biosynthesis K locus. The CPS was isolated from the strain and studied by sugar analysis, Smith degradation, one- and two-dimensional 1H and 13C NMR spectroscopy. It was composed of branched pentasaccharide units that include a →3)-α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc-(1→ mainchain and α-d-ManpNAc-(1 → 3)-l-Rhap side branch. Though the pentasaccharide units are identical to those that make up the K84 CPS produced by A. baumannii LUH5540, the units are linked differently via the substitution of an alternate l-Rhap residue, resulting in a difference in the overall topology of the CPS. This was due to the replacement of the Wzy polymerase gene encoded at the K locus.

Arbatsky N.P. , Shashkov A.S. , Shneider M.M. , Mikhailova Y.V. , Shelenkov A.A. , Sheck E.A. , Kasimova A.A. , Kalinchuk N.A. , Kenyon J.J. , Knirel Y.A.
The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15-4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology
Carbohydrate Research. 2024. V.545. 109273 . DOI: 10.1016/j.carres.2024.109273 WOS Scopus OpenAlex

Web of science:	WOS:001325078400001
Scopus:	2-s2.0-85204600652
OpenAlex:	W4402574014

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