Structure of the K58 capsular polysaccharide produced by Acinetobacter baumannii isolate MRSN 31468 includes Pse5Ac7Ac that is 4-O-acetylated by a phage-encoded acetyltransferase Научная публикация
Журнал |
Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215 |
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Вых. Данные | Год: 2024, Том: 547, Номер статьи : 109324, Страниц : DOI: 10.1016/j.carres.2024.109324 | ||||||||||||||||
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Реферат:
Capsular polysaccharide (CPS), a heteropolymeric carbohydrate structure present on the cell surface of most isolates of the bacterial pathogen Acinetobacter baumannii, is a major virulence determinant. Here, the CPS produced by A. baumannii MRSN 31468, which carries the KL58 CPS biosynthesis locus, was studied by sugar analysis, one- and two-dimensional 1H and 13C NMR spectroscopy. The structure was found to consist of a repeating tetrasaccharide K-unit that includes glucose (d-Glcp), galactose (d-Galp), N-acetyl-galactosamine (d-GalpNAc), and 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (5,7-di-N-acetylpseudaminic acid; Pse5Ac7Ac). The CPS has a branched repeating unit with the disaccharide →3)-β-d-Glc-(1→3)-β-d-GalNAc-(1→ as the mainchain and O-6 of the Glc unit substituted with the disaccharide β-Pse5Ac7Ac-(2→6)-α-d-Gal, and Pse5Ac7Ac is partially acetylated at O-4. The presence of Pse5Ac7Ac in the K58 structure is consistent with the presence of psaA-F genes in KL58, which are responsible for Pse5Ac7Ac synthesis. 4-O-acetylation of Pse5Ac7Ac was traced to an acetyltransferase, Atr44, which was found to be closely related to Atr29 that similarly decorates Pse5Ac7Ac with 4OAc in the K46-type CPS. Atr44 like Atr29 is encoded by a gene found in a prophage. The K58 CPS produced by MRSN 31468 did not include the 8-epimer of Pse5Ac7Ac (5,7-di-N-acetyl-8-epipseudaminic acid; 8ePse5Ac7Ac) found in the closely related CPS from BAL062 that also carries KL58. Hence, the gene(s) for conversion of Pse5Ac7Ac to 8ePse5Ac7Ac must lie elsewhere.
Библиографическая ссылка:
Iovine A.
, Filatov A.V.
, Kasimova A.A.
, Sharar N.S.
, Ambrose S.J.
, Dmitrenok A.S.
, Shneider M.M.
, Shpirt A.M.
, Perepelov A.V.
, Knirel Y.A.
, Hall R.M.
, De Castro C.
, Kenyon J.J.
Structure of the K58 capsular polysaccharide produced by Acinetobacter baumannii isolate MRSN 31468 includes Pse5Ac7Ac that is 4-O-acetylated by a phage-encoded acetyltransferase
Carbohydrate Research. 2024. V.547. 109324 . DOI: 10.1016/j.carres.2024.109324 WOS OpenAlex
Structure of the K58 capsular polysaccharide produced by Acinetobacter baumannii isolate MRSN 31468 includes Pse5Ac7Ac that is 4-O-acetylated by a phage-encoded acetyltransferase
Carbohydrate Research. 2024. V.547. 109324 . DOI: 10.1016/j.carres.2024.109324 WOS OpenAlex
Идентификаторы БД:
Web of science: | WOS:001364791700001 |
OpenAlex: | W4404528288 |
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