Complete chemical structure of the K135 capsular polysaccharide produced by Acinetobacter baumannii RES-546 that contains 5,7-di-N-acetyl-8-epipseudaminic acid

Complete chemical structure of the K135 capsular polysaccharide produced by Acinetobacter baumannii RES-546 that contains 5,7-di-N-acetyl-8-epipseudaminic acid Научная публикация

Журнал

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Вых. Данные

Год: 2023, Том: 523, Номер статьи : 108726, Страниц : DOI: 10.1016/j.carres.2022.108726

Авторы

Shashkov Alexander S. ¹ , Kasimova Anastasiya A. ¹ , Arbatsky Nikolay P. ¹ , Senchenkova Sof'ya N. ¹ , Perepelov Andrei V. ¹ , Dmitrenok Andrei S. ¹ , Chizhov Alexander O. ¹ , Knirel Yuriy A. ¹ , Shneider Mikhail M. ² , Popova Anastasia V. ³ , Kenyon Johanna J. ⁴

Организации

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	M.M. Shemyakin & Y. A Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow, Region, Russia
4	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia

A structurally diverse capsular polysaccharide (CPS) in the outer cell envelope plays an important role in the virulence of the important bacterial pathogen, Acinetobacter baumannii. More than 75 different CPS structures have been determined for the species to date, and many CPSs include isomers of a higher sugar, namely 5,7-diamino-3,5,7,9-tetradeoxynon-2-ulosonic acid. Recently, a novel isomer having the d-glycero-l-manno configuration (5,7-di-N-acetyl-8-epipseudaminic acid; 8ePse5Ac7Ac) has been identified in the CPS from A. baumannii clinical isolate RES-546 [Carbohydr. Res. 513 (2022) 108,531]. Here, the complete chemical structure of this CPS, designated K135, was elucidated. The CPS was found to have a branched tetrasaccharide K unit and to include the higher sugar as part of a 8ePse5Ac7Ac-(2 → 6)-α-Gal disaccharide branching from a →3)-α-D-GlcpNAc-(1 → 3)-β-D-GlcpNAc-(1→ main chain. Assignment of glycosyltransferases encoded by the CPS biosynthesis gene cluster in the RES-546 genome enabled the first sugar of the K unit, and hence the topology of the K135 CPS, to be determined.

Shashkov A.S. , Kasimova A.A. , Arbatsky N.P. , Senchenkova S.N. , Perepelov A.V. , Dmitrenok A.S. , Chizhov A.O. , Knirel Y.A. , Shneider M.M. , Popova A.V. , Kenyon J.J.
Complete chemical structure of the K135 capsular polysaccharide produced by Acinetobacter baumannii RES-546 that contains 5,7-di-N-acetyl-8-epipseudaminic acid
Carbohydrate Research. 2023. V.523. 108726 . DOI: 10.1016/j.carres.2022.108726 WOS Scopus OpenAlex

Web of science:	WOS:000891775300007
Scopus:	2-s2.0-85142699357
OpenAlex:	W4309725008

БД	Цитирований
OpenAlex	6
Scopus	2
Web of science	4