Structure of a 2‐aminoethyl phosphate‐containing O‐specific polysaccharide of Proteus penneri 63 from a new serogroup O68

Structure of a 2‐aminoethyl phosphate‐containing O‐specific polysaccharide of Proteus penneri 63 from a new serogroup O68 Full article

Journal

European Journal of Biochemistry
ISSN: 0014-2956 , E-ISSN: 0945-5795

Output data

Year: 2000, Volume: 267, Number: 2, Pages: 601-605 Pages count : 5 DOI: 10.1046/j.1432-1327.2000.01041.x

Authors

Shashkov Aleksander S. ¹ , Kondakova Anna N. ¹ , Senchenkova Sof’ya N. ¹ , Zych Krystyna ² , Toukach Filip V. ¹ , Knirel Yuriy A. ¹ , Sidorczyk Zygmunt ²

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Institute of Microbiology and Immunology, University of Lodz, Poland

Lipopolysaccharide of Proteus penneri strain 63 was degraded by mild acid to give a high molecular mass O-specific polysaccharide that was isolated by gel-permeation chromatography. Sugar and methylation analyses and NMR spectroscopic studies, including two-dimensional 1H,1H COSY, TOCSY rotating-frame NOE spectroscopy, H-detected 1H,13C and 1H,31P heteronuclear multiple-quantum coherence (HMQC), and 1H,13C HMQC-TOCSY experiments, demonstrated the following structure of the polysaccharide: where FucNAc is 2-acetamido-2,6-dideoxygalactose and PEtn is 2-aminoethyl phosphate. The polysaccharide studied shares some structural features, such as the presence of d-GlcNAc6PEtn and an α-L-FucNAc-(1→3)-d-GlcNAc disaccharide, with other Proteus O-specific polysaccharides. A marked cross-reactivity of P. penneri 63 O-antiserum with P. vulgaris O12 was observed and substantiated by a structural similarity of the O-specific polysaccharides of the two strains. In spite of this, the polysaccharide of P. penneri 63 has the unique structure among Proteus O-antigens, and therefore a new, separate serogroup, O68, is proposed for this strain.

Shashkov A.S. , Kondakova A.N. , Senchenkova S.N. , Zych K. , Toukach F.V. , Knirel Y.A. , Sidorczyk Z.
Structure of a 2‐aminoethyl phosphate‐containing O‐specific polysaccharide of Proteus penneri 63 from a new serogroup O68
European Journal of Biochemistry. 2000. V.267. N2. P.601-605. DOI: 10.1046/j.1432-1327.2000.01041.x OpenAlex

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