Structural and serological studies of the related O‐specific polysaccharides of Proteus vulgaris O21 and Proteus mirabilis O48 having oligosaccharide‐phosphate repeating units

Structural and serological studies of the related O‐specific polysaccharides of Proteus vulgaris O21 and Proteus mirabilis O48 having oligosaccharide‐phosphate repeating units Full article

Journal

European Journal of Biochemistry
ISSN: 0014-2956 , E-ISSN: 0945-5795

Output data

Year: 2000, Volume: 267, Number: 23, Pages: 6888-6896 Pages count : 9 DOI: 10.1046/j.1432-1033.2000.01793.x

Authors

Bartodziejska Beata ¹ , Toukach Filip V. ² , Vinogradov Evgeny V. ³ , Senchenkova Sofia N. ² , Shashkov Alexander S. ² , Ziolkowski Andrzej ¹ , Czaja Jolanta ⁴ , Perry Malcolm B. ³ , Knirel Yuriy A. ² , Rozalski Antoni ¹

Affiliations

1	Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
3	Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada
4	L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland

The O-specific polysaccharide chains (O-antigens) of the lipopolysaccharides (LPSs) of Proteus mirabilis O48 and Proteus vulgaris O21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. Polysaccharides and an oligosaccharide were derived from the LPSs by various degradation procedures and studied by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H,13C and 1H,31P HMQC experiments. The following related structures of the repeating units of the O-antigens were established (top: Proteus mirabilis O48; bottom: Proteus vulgaris O21) The O-specific polysaccharide of P. vulgaris O21 has the same structure as that of Hafnia allvei 744 and PCM 1194 [Petersson C., Jachymek, W., Klonowska, A., Lugowski, C., Niedziela, T. & Kenne, L. (1997) Eur. J. Biochem., 245, 668–675], except that the GlcN residue carries the N-acetyl rather than the N-[(R)-3-hydroxybutyryl] group. Serological investigations confirmed the close relatedness of the Proteus and Hafnia O-antigens studied.

Bartodziejska B. , Toukach F.V. , Vinogradov E.V. , Senchenkova S.N. , Shashkov A.S. , Ziolkowski A. , Czaja J. , Perry M.B. , Knirel Y.A. , Rozalski A.
Structural and serological studies of the related O‐specific polysaccharides of Proteus vulgaris O21 and Proteus mirabilis O48 having oligosaccharide‐phosphate repeating units
European Journal of Biochemistry. 2000. V.267. N23. P.6888-6896. DOI: 10.1046/j.1432-1033.2000.01793.x OpenAlex

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