Structure of Klebsiella grimontii K15g Capsular Polysaccharide and Products of Its Cleavage by Phage Silvester Depolymerase

Structure of Klebsiella grimontii K15g Capsular Polysaccharide and Products of Its Cleavage by Phage Silvester Depolymerase Научная публикация

Журнал

Biochemistry-Moscow
ISSN: 0006-2979 , E-ISSN: 1608-3040

Вых. Данные

Год: 2026, Том: 91, Номер: 1, Страницы: 81-94 Страниц : 14 DOI: 10.1134/s0006297925601935

Авторы

Lukianova Anna A. ¹ , Tokmakova Anna D. ^2,1 , Kasimova Anastasia A. ³ , Shelenkov Andrey A. ⁴ , Mikhailova Yuliya V. ⁴ , Putilov Ilia A. ^5,1 , Knirel Yuriy A. ³ , Miroshnikov Konstantin A. ¹ , Shneider Mikhail M. ¹

Организации

1	Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, 117997, Moscow, Russia
2	Moscow Center for Advanced Studies, 123592, Moscow, Russia
3	Zelinsky Institute of Organic Chemistry, Russian Academy of Science, 119991, Moscow, Russia
4	Central Research Institute of Epidemiology, Rospotrebnadzor, 111123, Moscow, Russia
5	National Research University Higher School of Economics, 101000, Moscow, Russia

Klebsiella grimontii is a recently described pathogen (former member of the Klebsiella oxytoca phylogroup K06), which has been elevated to the rank of a new species. The capsular polysaccharide (CPS) of K. grimontii is a principal virulence factor that significantly enhances the capacity of the bacterium to induce infections. The structure of CPS from K. grimontii strain K15g determined is this study was found to represent a novel type of polysaccharide consisting of branched hexasaccharide K-units, each composed of four monosaccharides of the main chain and a disaccharide side chain containing pyruvic acid 4,6-acetal residues. Since the CPS cleavage initiates the infection of bacterial cells by a phage, we investigated the depolymerase activity of the phage Silvester structural protein and demonstrated that the recombinant depolymerase of the phage Silvester cleaved K. grimontii K15g CPS at the β-D-GlcpA-(1→2)-α-L-Rhap linkage between the repeating units. The pathway for the synthesis of CPS of this type was proposed.

Lukianova A.A. , Tokmakova A.D. , Kasimova A.A. , Shelenkov A.A. , Mikhailova Y.V. , Putilov I.A. , Knirel Y.A. , Miroshnikov K.A. , Shneider M.M.
Structure of Klebsiella grimontii K15g Capsular Polysaccharide and Products of Its Cleavage by Phage Silvester Depolymerase
Biochemistry-Moscow. 2026. V.91. N1. P.81-94. DOI: 10.1134/s0006297925601935 WOS Scopus OpenAlex

Поступила в редакцию:	28 июл. 2025 г.
Опубликована online:	10 февр. 2026 г.

Web of science:	WOS:001686377400002
Scopus:	2-s2.0-105029629133
OpenAlex:	W7128474617

БД	Цитирований
OpenAlex	Нет цитирований
Scopus	Нет цитирований