Structure of the O-specific polysaccharide from the lipopolysaccharide of Azospirillum brasilense KR77

Structure of the O-specific polysaccharide from the lipopolysaccharide of Azospirillum brasilense KR77 Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2026, Volume: 567, Article number : 109988, Pages count : DOI: 10.1016/j.carres.2026.109988

Authors

Sigida Elena N. ^1,2 , Toukach Philip V. ² , Zdorovenko Evelina L. ² , Dmitrenok Andrei S. ² , Kuzina Marina S. ¹ , Konnova Svetlana A. ^1,3 , Fedonenko Yuliya P. ¹

Affiliations

1	Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov 410049, Russia
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, Moscow 119991, Russia
3	Saratov State University, Ulitsa Astrakhanskaya 83, 410012, Saratov, Russia

Azospirillum brasilense is a plant-growth-promoting rhizobacterium widely distributed in associations with crops. Lipopolysaccharide was extracted by Westphal procedure from A. brasilense KR77, isolated in Senegal. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in O-specific polysaccharide, which was studied by compositional analysis, 1D and 2D 1H and 13C NMR spectroscopy along with computational NMR-based structure analysis, and Smith degradation. The following structure of heptasaccharide repeating units was established: →2)-[α-d-Galf-(1→2)-α-l-Rhap-(1→3)-α-l-Rhap-(1→3)-α-l-Rhap(1→3)]-β-d-Glcp-(1→3)-α-l-Fucp-(1→3)-β-d-GlcpNAc-(1 → .

Sigida E.N. , Toukach P.V. , Zdorovenko E.L. , Dmitrenok A.S. , Kuzina M.S. , Konnova S.A. , Fedonenko Y.P.
Structure of the O-specific polysaccharide from the lipopolysaccharide of Azospirillum brasilense KR77
Carbohydrate Research. 2026. V.567. 109988 . DOI: 10.1016/j.carres.2026.109988 WOS Scopus OpenAlex

Submitted:	Apr 28, 2026
Published online:	May 30, 2026

≡ Web of science:	WOS:001786946100001
≡ Scopus:	2-s2.0-105040205290
≡ OpenAlex:	W7162689835