Structure elucidation and gene cluster annotation of the O-antigen of Stutzerimonas chloritidismutans NP21570 with 2-acetamido-2-deoxy-D-mannuronic acid

Structure elucidation and gene cluster annotation of the O-antigen of Stutzerimonas chloritidismutans NP21570 with 2-acetamido-2-deoxy-D-mannuronic acid Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2026, Volume: 567, Article number : 109993, Pages count : DOI: 10.1016/j.carres.2026.109993

Authors

Perepelov Andrei V. ¹ , Kasimova Anastasiya A. ¹ , Filatov Andrei V. ¹ , Toukach Philip V. ¹ , Babich Tamara L. ² , Popova Nadezhda M. ³ , Krivonos Danil V. ^4,5 , Safonov Alexey V. ⁶

Affiliations

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
2	Research Center of Biotechnology, Russian Academy of Sciences, 7, bld. 2, 60 let Oktjabrja pr-t, Moscow, 117312, Russian Federation
3	A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 31/4, Leninsky Ave., Moscow, 199071, Russian Federation
4	Research Institute for Systems Biology and Medicine (RISBM), 18, Nauchniy proezd, Moscow, 117246, Russian Federation
5	Moscow Center for Advanced Studies, 20, Kulakova Str., Moscow, Russian Federation
6	V. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Kosygina Str. 19, Moscow, Russian Federation. Electronic address: alexeysafonof@gmail.com

The O-polysaccharide (O-antigen) of Stutzerimonas chloritidismutans NP21570 isolated from deep saline aquifer (1570 m) near Dimitrovgrad, Russian Federation, was studied by chemical methods, including component analyses, along with 1H and 13C NMR spectroscopy and NMR-based structure matching. The following structure of linear disaccharide repeating O-unit, containing two O-acetyl groups, one of them is about 40%, another one is 60%, was established.

Perepelov A.V. , Kasimova A.A. , Filatov A.V. , Toukach P.V. , Babich T.L. , Popova N.M. , Krivonos D.V. , Safonov A.V.
Structure elucidation and gene cluster annotation of the O-antigen of Stutzerimonas chloritidismutans NP21570 with 2-acetamido-2-deoxy-D-mannuronic acid
Carbohydrate Research. 2026. V.567. 109993 . DOI: 10.1016/j.carres.2026.109993 WOS Scopus OpenAlex

Submitted:	Apr 3, 2026
Accepted:	May 29, 2026
Published online:	Jun 5, 2026

≡ Web of science:	WOS:001794965400001
≡ Scopus:	2-s2.0-105041162787
≡ OpenAlex:	W7163656906