The Acinetobacter baumannii K70 and K9 capsular polysaccharides consist of related K-units linked by the same Wzy polymerase and cleaved by the same phage depolymerases

The Acinetobacter baumannii K70 and K9 capsular polysaccharides consist of related K-units linked by the same Wzy polymerase and cleaved by the same phage depolymerases Научная публикация

Журнал

Microbiology spectrum
ISSN: 2165-0497

Вых. Данные

Год: 2023, Том: 11, Номер: 6, Номер статьи : e03025-23, Страниц : DOI: 10.1128/spectrum.03025-23

Авторы

Kasimova Anastasiya A. ¹ , Sharar Nowshin S. ² , Ambrose Stephanie J. ³ , Knirel Yuriy A. ¹ , Shneider Mikhail M. ⁴ , Timoshina Olga Y. ⁴ , Popova Anastasiya V. ⁵ , Perepelov Andrey V. ¹ , Dmitrenok Andrey S. ¹ , Hsu Li Yang ⁶ , Hall Ruth M. ³ , Kenyon Johanna J. ²

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
3	School of Life and Environmental Sciences, Faculty of Science, University of Sydney, Sydney, Australia
4	M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
6	Saw Swee Hock School of Public Health, National University of Singapore, Queenstown, Singapore

The extensively antibiotic-resistant Acinetobacter baumannii GC1(ST1IP) isolate SGH0807 from Singapore carries the KL70 gene cluster. The structure of the K70 capsular polysaccharide (CPS) produced by SGH0807 was determined using sugar analysis and one- and two-dimensional 1H and 13C NMR spectroscopy. The K70 CPS consists of branched tetrasaccharide K-units and is closely related to the previously reported K9 CPS. The KL70 and KL9 loci differ in a short segment that encodes the initiating transferase for d-FucpNAc in K70 and d-GlcpNAc in K9. The two structures differ only in the identity of the “first” sugar of the K-unit, d-FucpNAc in K70 and d-GlcpNAc in K9. This difference alters the identity of one of the sugars involved in the linkage between K-units formed by the Wzy polymerase. However, KL70 and KL9 encode an identical Wzy polymerase, designated as WzyKL9, indicating that the differences between d-FucpNAc and d-GlcpNAc do not affect its function. Whether the difference in the first sugars was recognized by the depolymerases encoded by three K9-specific bacteriophages, AM24, BS46, and APK09, that hydrolyze the bond in K9 CPS formed by WzyKL9 was also examined. Purified depolymerases incubated with K70 CPS purified from SGH0807 formed oligosaccharide fragments that were monomers and dimers of the CPS cleaved at the linkage between K-units. As depolymerases encoded by phage determine host specificity by hydrolyzing specific CPS types, these phages could infect and lyse the SGH0807 K70 isolate. A. baumannii carrying KL70 were found in Singapore hospitals between 2006 and 2009.

Kasimova A.A. , Sharar N.S. , Ambrose S.J. , Knirel Y.A. , Shneider M.M. , Timoshina O.Y. , Popova A.V. , Perepelov A.V. , Dmitrenok A.S. , Hsu L.Y. , Hall R.M. , Kenyon J.J.
The Acinetobacter baumannii K70 and K9 capsular polysaccharides consist of related K-units linked by the same Wzy polymerase and cleaved by the same phage depolymerases
Microbiology spectrum. 2023. V.11. N6. e03025-23 . DOI: 10.1128/spectrum.03025-23 WOS Scopus OpenAlex

Web of science:	WOS:001107180000001
Scopus:	2-s2.0-85180005004
OpenAlex:	W4388762306

БД	Цитирований
OpenAlex	4
Scopus	4