Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin

Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin Full article

Journal

Frontiers in Microbiology
ISSN: 1664-302X

Output data

Year: 2022, Volume: 13, Article number : 1003942, Pages count : DOI: 10.3389/fmicb.2022.1003942

Authors

Gannesen Andrei V. ¹ , Ziganshin Rustam H. ² , Zdorovenko Evelina L. ³ , Klimko Alena I. ⁴ , Ianutsevich Elena A. ¹ , Danilova Olga A. ¹ , Tereshina Vera M. ¹ , Gorbachevskii Maxim V. ⁵ , Ovcharova Maria A. ¹ , Nevolina Ekaterina D. ¹ , Martyanov Sergey V. ¹ , Shashkov Alexander S. ³ , Dmitrenok Andrey S. ³ , Novikov Andrei A. ⁵ , Zhurina Marina V. ¹ , Botchkova Ekaterina A. ^5,1 , Toukach Philipp V. ³ , Plakunov Vladimir K. ¹

Affiliations

1	Winogradsky Institute of Microbiology, Federal Research Center “Fundamentals of Biotechnology” of Russian Academy of Sciences, Moscow, Russia
2	Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
4	Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia
5	Faculty of Chemical and Environmental Engineering, Gubkin University, Moscow, Russia

The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth. Epinephrine in concentration 4.9 × 10–9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition. The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone. One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation. The matrix phospholipids were also eradicated by the addition of the hormone. Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.

Gannesen A.V. , Ziganshin R.H. , Zdorovenko E.L. , Klimko A.I. , Ianutsevich E.A. , Danilova O.A. , Tereshina V.M. , Gorbachevskii M.V. , Ovcharova M.A. , Nevolina E.D. , Martyanov S.V. , Shashkov A.S. , Dmitrenok A.S. , Novikov A.A. , Zhurina M.V. , Botchkova E.A. , Toukach P.V. , Plakunov V.K.
Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin
Frontiers in Microbiology. 2022. V.13. 1003942 . DOI: 10.3389/fmicb.2022.1003942 WOS Scopus OpenAlex

≡ Web of science:	WOS:000874171600001
≡ Scopus:	2-s2.0-85139228741
≡ OpenAlex:	W4297906695