Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal

Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal Научная публикация

Журнал

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Вых. Данные

Год: 2022, Том: 218, Страницы: 447-455 Страниц : 9 DOI: 10.1016/j.ijbiomac.2022.07.136

Авторы

Kasimova Anastasiya A. ¹ , Shneider Mikhail M. ² , Edelstein Mikhail V. ³ , Dzhaparova Alina A. ¹ , Shashkov Alexander S. ¹ , Knirel Yuriy A. ¹ , Kenyon Johanna J. ⁴

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	M. M. Shemyakin and Y. A.Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997 Moscow, Russia
3	Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia
4	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia

The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units) that include one residue each of d-GlcpNAc, d-GalpNAc, 2-acetamido-2-deoxy-d-galacturonic acid (d-GalpNAcA), and 2-acetamido-2,6-dideoxy-d-glucose (N-acetylquinovosamine, d-QuipNAc), with the GalpNAc residue decorated with a (R)-configurated 4,6-pyruvic acid acetal group. The CPS has a similar composition to that of A. baumannii K4 but the topology of the tetrasaccharide K-unit is different (linear in K98 versus branched in K4). This was due to a difference in sequence for the Wzy polymerases encoded by the CPS biosynthesis gene clusters KL98 and KL4, with the WzyK98 polymerase forming a β-d-QuipNAc-(1→3)-d-GalpNAc linkage between the K98 units.

Kasimova A.A. , Shneider M.M. , Edelstein M.V. , Dzhaparova A.A. , Shashkov A.S. , Knirel Y.A. , Kenyon J.J.
Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal
International Journal of Biological Macromolecules. 2022. V.218. P.447-455. DOI: 10.1016/j.ijbiomac.2022.07.136 WOS Scopus OpenAlex

Web of science:	WOS:000877210000002
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