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Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal Научная публикация

Журнал International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130
Вых. Данные Год: 2022, Том: 218, Страницы: 447-455 Страниц : 9 DOI: 10.1016/j.ijbiomac.2022.07.136
Авторы Kasimova Anastasiya A. 1 , Shneider Mikhail M. 2 , Edelstein Mikhail V. 3 , Dzhaparova Alina A. 1 , Shashkov Alexander S. 1 , Knirel Yuriy A. 1 , Kenyon Johanna J. 4
Организации
1 N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2 M. M. Shemyakin and Y. A.Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997 Moscow, Russia
3 Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia
4 Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia

Реферат: The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units) that include one residue each of d-GlcpNAc, d-GalpNAc, 2-acetamido-2-deoxy-d-galacturonic acid (d-GalpNAcA), and 2-acetamido-2,6-dideoxy-d-glucose (N-acetylquinovosamine, d-QuipNAc), with the GalpNAc residue decorated with a (R)-configurated 4,6-pyruvic acid acetal group. The CPS has a similar composition to that of A. baumannii K4 but the topology of the tetrasaccharide K-unit is different (linear in K98 versus branched in K4). This was due to a difference in sequence for the Wzy polymerases encoded by the CPS biosynthesis gene clusters KL98 and KL4, with the WzyK98 polymerase forming a β-d-QuipNAc-(1→3)-d-GalpNAc linkage between the K98 units.
Библиографическая ссылка: Kasimova A.A. , Shneider M.M. , Edelstein M.V. , Dzhaparova A.A. , Shashkov A.S. , Knirel Y.A. , Kenyon J.J.
Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal
International Journal of Biological Macromolecules. 2022. V.218. P.447-455. DOI: 10.1016/j.ijbiomac.2022.07.136 WOS Scopus OpenAlex
Идентификаторы БД:
Web of science: WOS:000877210000002
Scopus: 2-s2.0-85135706372
OpenAlex: W4286457422
Цитирование в БД:
БД Цитирований
OpenAlex 3
Scopus 3
Web of science 3
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