Реферат: Great progresses have been made in the recent years in the detection of circulating galactofuranose-bearing molecules for the diagnosis of aspergillosis. However, the test used in the clinical practice is hampered by the occurrence of false positives. A glycoarray with dozens of oligosaccharides structurally related to the Aspergillus fumigatus galactomannan has allowed us to reinvestigate the carbohydrate specificity of the EB-A2 monoclonal antibody used in the PlateliaTM Aspergillus sandwich immune assay. We have now demonstrated that the mAb can recognize shorter oligosaccharides than the previously reported tetrasaccharide Galf-β-(1→5)-Galf-β-(1→5)-Galf-β-(1→5)-Galf-β and oligosaccharides which contains alternating β-(1→5)/β-(1→6)-linkages. This result could explain the occurrence of false-positive signals due to the presence of the abovementioned epitopes not only in A. fumigatus galactomannan but also in other bacteria and fungi.

Библиографическая ссылка: Krylov V.B. , Solovev A.S. , Argunov D.A. , Latgé J-P. , Nifantiev N.E.
Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan
Heliyon. 2019. V.5. N1. e01173 . DOI: 10.1016/j.heliyon.2019.e01173 WOS Scopus OpenAlex

Идентификаторы БД:

≡ Web of science:	WOS:000460080900064
≡ Scopus:	2-s2.0-85060769509
≡ OpenAlex:	W2911795775

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