Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan Full article
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Heliyon
ISSN: 2405-8440 |
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| Output data | Year: 2019, Volume: 5, Number: 1, Article number : e01173, Pages count : DOI: 10.1016/j.heliyon.2019.e01173 | ||||
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Abstract:
Great progresses have been made in the recent years in the detection of circulating galactofuranose-bearing molecules for the diagnosis of aspergillosis. However, the test used in the clinical practice is hampered by the occurrence of false positives. A glycoarray with dozens of oligosaccharides structurally related to the Aspergillus fumigatus galactomannan has allowed us to reinvestigate the carbohydrate specificity of the EB-A2 monoclonal antibody used in the PlateliaTM Aspergillus sandwich immune assay. We have now demonstrated that the mAb can recognize shorter oligosaccharides than the previously reported tetrasaccharide Galf-β-(1→5)-Galf-β-(1→5)-Galf-β-(1→5)-Galf-β and oligosaccharides which contains alternating β-(1→5)/β-(1→6)-linkages. This result could explain the occurrence of false-positive signals due to the presence of the abovementioned epitopes not only in A. fumigatus galactomannan but also in other bacteria and fungi.
Cite:
Krylov V.B.
, Solovev A.S.
, Argunov D.A.
, Latgé J-P.
, Nifantiev N.E.
Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan
Heliyon. 2019. V.5. N1. e01173 . DOI: 10.1016/j.heliyon.2019.e01173 WOS Scopus OpenAlex
Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan
Heliyon. 2019. V.5. N1. e01173 . DOI: 10.1016/j.heliyon.2019.e01173 WOS Scopus OpenAlex
Identifiers:
| ≡ Web of science: | WOS:000460080900064 |
| ≡ Scopus: | 2-s2.0-85060769509 |
| ≡ OpenAlex: | W2911795775 |