Sciact
  • EN
  • RU

The structure of Klebsiella pneumoniae K108 capsular polysaccharide is similar to Escherichia coli colanic acid Научная публикация

Журнал International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130
Вых. Данные Год: 2023, Том: 244, Номер статьи : 125403, Страниц : DOI: 10.1016/j.ijbiomac.2023.125403
Авторы Kasimova Anastasiya A. 1 , Shneider Mikhail M. 2 , Evseev Peter V. 2 , Shelenkov Andrey A. 3 , Mikhailova Yuliya V. 3 , Miroshnikov Konstantin A. 2 , Chebotar Igor V. 4 , Shagin Dmitry A. 4
Организации
1 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Science, Moscow, Russia
2 M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia
3 Central Research Institute of Epidemiology, Moscow, Russia
4 Pirogov Russian National Research Medical University, Moscow, Russia

Реферат: The clinical isolate of Klebsiella pneumoniae 1333/P225 was revealed as containing a KL108 K. pneumoniae K locus for capsule biosynthesis. The gene cluster demonstrated a high level of sequence and arrangement similarity with that of the E. coli colanic acid biosynthesis gene cluster. The KL108 gene cluster includes a gene of WcaD polymerase responsible for joining oligosaccharide K units into capsular polysaccharide (CPS), acetyltransferase, pyruvyltransferasefive and genes for glycosyltransferases (Gtrs), four of which have homologues in genetic units of the colanic acid synthesis. The fifth Gtr is specific to this cluster. The work involved the use of sugar analysis, Smith degradation and one- and two-dimensional 1H and 13C NMR spectroscopy to establish the structure of the K108 CPS. The CPS repetitive K unit is composed of branched pentasaccharide with three monosaccharides in the backbone and a disaccharide side chain. The main chain is the same as for colanic acid but the side chain differs. Two bacteriophages infecting K. pneumoniae strain 1333/P225 were isolated and structural depolymerase genes were determined; depolymerases Dep108.1 and Dep108.2 were cloned, expressed and purified. It was demonstrated that both depolymerases specifically cleave the β-Glcp-(1→4)-α-Fucp linkage between K108 units in the CPS.
Библиографическая ссылка: Kasimova A.A. , Shneider M.M. , Evseev P.V. , Shelenkov A.A. , Mikhailova Y.V. , Miroshnikov K.A. , Chebotar I.V. , Shagin D.A.
The structure of Klebsiella pneumoniae K108 capsular polysaccharide is similar to Escherichia coli colanic acid
International Journal of Biological Macromolecules. 2023. V.244. 125403 . DOI: 10.1016/j.ijbiomac.2023.125403 WOS Scopus OpenAlex
Идентификаторы БД:
Web of science: WOS:001090440900001
Scopus: 2-s2.0-85163192218
OpenAlex: W4380758048
Цитирование в БД:
БД Цитирований
OpenAlex 1
Web of science 1
Альметрики: