Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020

Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020 Full article

Journal

PLOS ONE
ISSN: 1932-6203

Output data

Year: 2021, Volume: 16, Number: 7, Article number : e0253482, Pages count : DOI: 10.1371/journal.pone.0253482

Authors

Liyaskina Elena V. ¹ , Rakova Nadezhda A. ¹ , Kitykina Alevtina A. ¹ , Rusyaeva Valentina V. ¹ , Toukach Philip V. ² , Fomenkov Alexey ³ , Vainauskas Saulius ³ , Roberts Richard J. ³ , Revin Victor V. ¹

Affiliations

1	Department of Biotechnology, Bioengineering and Biochemistry of the National Research Mordovia State University, Saransk, Russia
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
3	New England Biolabs Inc., Ipswich, MA, United States of America

Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolated from wasp honeycombs. Here we report its complete genome sequence and full methylome analysis detected by Pacific Biosciences SMRT sequencing. Moreover, bioinformatic analysis identified a putative levan synthetic operon. SacC and sacB genes have been cloned and their products identified as glycoside hydrolase and levansucrase respectively. The Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectra demonstrated that the EPS is a linear β-(2→6)-linked fructan (levan). The structure and properties of levan polymer produced from sucrose and molasses were analyzed by FT-IR, NMR, scanning electron microscopy (SEM), high performance size exclusion chromatography (HPSEC), thermogravimetric analysis (TGA), cytotoxicity tests and showed low toxicity and high biocompatibility. Thus, P. polymyxa 2020 could be an exceptional cost-effective source for the industrial production of levan-type EPSs and to obtain functional biomaterials based on it for a broad range of applications, including bioengineering.

Liyaskina E.V. , Rakova N.A. , Kitykina A.A. , Rusyaeva V.V. , Toukach P.V. , Fomenkov A. , Vainauskas S. , Roberts R.J. , Revin V.V.
Production and сharacterization of the exopolysaccharide from strain Paenibacillus polymyxa 2020
PLOS ONE. 2021. V.16. N7. e0253482 . DOI: 10.1371/journal.pone.0253482 WOS Scopus OpenAlex

≡ Web of science:	WOS:000674293700027
≡ Scopus:	2-s2.0-85109281421
≡ OpenAlex:	W3179203519