The Acinetobacter baumannii K239 capsular polysaccharide includes heptasaccharide units that are structurally related to K86 but joined by different linkages formed by different Wzy polymerases

The Acinetobacter baumannii K239 capsular polysaccharide includes heptasaccharide units that are structurally related to K86 but joined by different linkages formed by different Wzy polymerases Full article

Journal

International Journal of Biological Macromolecules
ISSN: 1879-0003 , E-ISSN: 0141-8130

Output data

Year: 2024, Article number : 130045, Pages count : DOI: 10.1016/j.ijbiomac.2024.130045

Authors

Kasimova Anastasiya A. ¹ , Shashkov Alexander S. ¹ , Shneider Mikhail M. ² , Sheck Eugenii A. ³ , Mikhailova Yulia V. ⁴ , Shelenkov Andrey A. ⁴ , Popova Anastasiya V. ⁵ , Knirel Yuriy A. ¹ , Kenyon Johanna J. ⁶

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	M. M. Shemyakin and Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 119997 Moscow, Russia
3	Institute of Antimicrobial Chemotherapy (IAC), Smolensk State Medical University (SSMU), Kirova St. 46a, Smolensk 214019, Russia
4	Central Scientific Research Institute for Epidemiology, Moscow, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia
6	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia

The K239 type capsular polysaccharide (CPS) isolated from Acinetobacter baumannii isolate MAR19-4435 was studied by sugar analysis, one- and two-dimensional 1H and 13C NMR spectroscopy. K239 consists of branched heptasaccharide repeats (K-units) comprised of five residues of l-rhamnose (l-Rhap), and one residue each of d-glucuronic acid (d-GlcpA) and N-acetyl-d-glucosamine (d-GlcpNAc). The structure of K239 is closely related to that of the A. baumannii K86 CPS type, though the two differ in the 2,3-substitution patterns on the l-Rhap residue that is involved in the linkage between K-units in the CPS polymer. This structural difference was attributed to the presence of a gtr221 glycosyltransferase gene and a wzyKL239 polymerase gene in KL239 that replaces the gtr80 and wzyKL86 genes in the KL86 CPS biosynthesis gene cluster. Comparison of the two structures established the role of a novel WzyKL239 polymerase encoded by KL239 that forms the β-d-GlcpNAc-(1→2)-l-Rhap linkage between K239 units. A. baumannii MAR19-4435 was found to be non-susceptible to infection by the APK86 bacteriophage, which encodes a depolymerase that specifically cleaves the linkage between K-units in the K86 CPS, indicating that the difference in 2,3-substitution of l-Rhap influences the susceptibility of this isolate to bacteriophage activity.

Kasimova A.A. , Shashkov A.S. , Shneider M.M. , Sheck E.A. , Mikhailova Y.V. , Shelenkov A.A. , Popova A.V. , Knirel Y.A. , Kenyon J.J.
The Acinetobacter baumannii K239 capsular polysaccharide includes heptasaccharide units that are structurally related to K86 but joined by different linkages formed by different Wzy polymerases
International Journal of Biological Macromolecules. 2024. 130045 . DOI: 10.1016/j.ijbiomac.2024.130045 WOS Scopus OpenAlex

Web of science:	WOS:001182394400001
Scopus:	2-s2.0-85184843935
OpenAlex:	W4391617102

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OpenAlex	2
Scopus	1
Web of science	2