Реферат: The K239 type capsular polysaccharide (CPS) isolated from Acinetobacter baumannii isolate MAR19-4435 was studied by sugar analysis, one- and two-dimensional 1H and 13C NMR spectroscopy. K239 consists of branched heptasaccharide repeats (K-units) comprised of five residues of l-rhamnose (l-Rhap), and one residue each of d-glucuronic acid (d-GlcpA) and N-acetyl-d-glucosamine (d-GlcpNAc). The structure of K239 is closely related to that of the A. baumannii K86 CPS type, though the two differ in the 2,3-substitution patterns on the l-Rhap residue that is involved in the linkage between K-units in the CPS polymer. This structural difference was attributed to the presence of a gtr221 glycosyltransferase gene and a wzyKL239 polymerase gene in KL239 that replaces the gtr80 and wzyKL86 genes in the KL86 CPS biosynthesis gene cluster. Comparison of the two structures established the role of a novel WzyKL239 polymerase encoded by KL239 that forms the β-d-GlcpNAc-(1→2)-l-Rhap linkage between K239 units. A. baumannii MAR19-4435 was found to be non-susceptible to infection by the APK86 bacteriophage, which encodes a depolymerase that specifically cleaves the linkage between K-units in the K86 CPS, indicating that the difference in 2,3-substitution of l-Rhap influences the susceptibility of this isolate to bacteriophage activity.

Библиографическая ссылка: Kasimova A.A. , Shashkov A.S. , Shneider M.M. , Sheck E.A. , Mikhailova Y.V. , Shelenkov A.A. , Popova A.V. , Knirel Y.A. , Kenyon J.J.
The Acinetobacter baumannii K239 capsular polysaccharide includes heptasaccharide units that are structurally related to K86 but joined by different linkages formed by different Wzy polymerases
International Journal of Biological Macromolecules. 2024. 130045 . DOI: 10.1016/j.ijbiomac.2024.130045 WOS Scopus OpenAlex

Идентификаторы БД:

Web of science:	WOS:001182394400001
Scopus:	2-s2.0-85184843935
OpenAlex:	W4391617102

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БД	Цитирований
OpenAlex	1
Scopus	1
Web of science	1

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