Abstract: A branched O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Yersinia rohdei H274-36/78 and found to contain d-rhamnose, d-mannose, and 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-d-xylo-hexose called yersiniose A (Yer). Partial acid hydrolysis of the O-polysaccharide eliminated Yer residues to give a modified linear polysaccharide. Studies by sugar analysis and 1H and 13C NMR spectroscopy, including computational NMR analysis, enabled structure elucidation of a hexasaccharide repeating unit of the O-polysaccharide having two Yer residues attached as monosaccharide side chains. The O-antigen gene cluster of Y. rohdei H274-36/78 located between JUMPStart and galF genes contained putative genes for synthesis of precursors of two O-antigen constituents, GDP-d-Man and GDP-d-Rha, whereas genes responsible for synthesis of CDP-Yer were within the chromosome outside the O-antigen gene cluster. Glycosyltransferase genes and ABC 2 transporter genes were present in the O-antigen gene cluster, and hence the structure established is consistent with the polysaccharide synthesis gene content of the genome.

Cite: Sizova O.V. , Shashkov A.S. , Dmitrenok A.S. , Toukach P.V. , Knirel Y.A. , Shaikhutdinova R.Z. , Ivanov S.A. , Kislichkina A.A. , Bogun A.G. , Dentovskaya S.V.
Structure and gene cluster of the O-polysaccharide of Yersinia rohdei H274-36/78
International Journal of Biological Macromolecules. 2019. V.122. P.555-561. DOI: 10.1016/j.ijbiomac.2018.10.189 WOS Scopus OpenAlex

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Web of science:	WOS:000456226700062
Scopus:	2-s2.0-85056155883
OpenAlex:	W2899363342

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