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Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens Full article

Journal Russian Journal of Bioorganic Chemistry
ISSN: 1608-330X , E-ISSN: 1573-9163
Output data Year: 2018, Volume: 44, Number: 1, Pages: 80-89 Pages count : 10 DOI: 10.1134/s1068162017060073
Authors Krylov V.B. 1 , Petruk M.I. 1 , Grigoryev I.V. 2 , Lebedin Y.S. 2 , Glushko N.I. 3 , Khaldeeva E.V. 3 , Argunov D.A. 1 , Khatuntseva E.A. 1 , Toplishek M.V. 1 , Komarova B.S. 1 , Karelin A.A. 1 , Yudina O.N. 1 , Menshov V.M. 1 , Yashunskii D.V. 1 , Tsvetkov Y.E. 1 , Nifantiev N.E. 1
Affiliations
1 Laboratory of Glycoconjugate Chemistry, Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2 XEMA Company Limited, Moscow, Russia
3 Kazan Scientific Research Institute of Epidemiology and Microbiology, Kazan, Russia

Abstract: The carbohydrate specificity of antibodies obtained by immunizing laboratory animals with immunogens prepared from the Aspergillus fumigatus cell wall was analyzed using the library of biotinylated synthetic oligosaccharides. It has been shown that the main part of antipolysaccharide antibodies recognizes galactomannan in the studied immunogens with preference to its fragments containing more than two β-(1→5)-linked galactofuranosyl units. The data obtained can form the basis for the development of enzyme immunoassay for the detection of this dangerous fungal pathogen.
Cite: Krylov V.B. , Petruk M.I. , Grigoryev I.V. , Lebedin Y.S. , Glushko N.I. , Khaldeeva E.V. , Argunov D.A. , Khatuntseva E.A. , Toplishek M.V. , Komarova B.S. , Karelin A.A. , Yudina O.N. , Menshov V.M. , Yashunskii D.V. , Tsvetkov Y.E. , Nifantiev N.E.
Study of the Carbohydrate Specificity of Antibodies Against Aspergillus fumigatus Using the Library of Synthetic Mycoantigens
Russian Journal of Bioorganic Chemistry. 2018. V.44. N1. P.80-89. DOI: 10.1134/s1068162017060073 WOS Scopus OpenAlex
Identifiers:
≡ Web of science: WOS:000427499200008
≡ Scopus: 2-s2.0-85043981680
≡ OpenAlex: W2792112977
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