Structure of the O-polysaccharide of Providencia alcalifaciens O8 containing (2S,4R)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans

Structure of the O-polysaccharide of Providencia alcalifaciens O8 containing (2S,4R)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2008, Volume: 343, Number: 15, Pages: 2706-2711 Pages count : 6 DOI: 10.1016/j.carres.2008.08.011

Authors

Toukach Filip V. ¹ , Kocharova Nina A. ¹ , Maszewska Agnieszka ² , Shashkov Alexander S. ¹ , Knirel Yuriy A. ¹ , Rozalski Antoni ²

Affiliations

1	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
2	Institute of Microbiology and Immunology, University of Lodz, PL 90-237 Lodz, Poland

A glycerol teichoic acid-like O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O8 and studied by chemical methods and NMR spectroscopy, including 2D ROESY, {1H,13C} HSQC, and HMQC-TOCSY experiments. It was found that the compound contains a new component of bacterial lipopolysaccharides: ether-linked (2S,4R)-2,4-dihydroxypentanoic acid (Dhpa), which was identified by NMR spectroscopy. The following structure of the repeating unit of the polysaccharide was established:

Toukach F.V. , Kocharova N.A. , Maszewska A. , Shashkov A.S. , Knirel Y.A. , Rozalski A.
Structure of the O-polysaccharide of Providencia alcalifaciens O8 containing (2S,4R)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans
Carbohydrate Research. 2008. V.343. N15. P.2706-2711. DOI: 10.1016/j.carres.2008.08.011 WOS Scopus OpenAlex

≡ Web of science:	WOS:000260211400028
≡ Scopus:	2-s2.0-51249122211
≡ OpenAlex:	W2075095058