Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides

Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides Научная публикация

Журнал

International Journal of Molecular Sciences
ISSN: 1661-6596 , E-ISSN: 1422-0067

Вых. Данные

Год: 2021, Том: 22, Номер: 11, Номер статьи : 5641, Страниц : DOI: 10.3390/ijms22115641

Авторы

Kasimova Anastasiya A. ¹ , Arbatsky Nikolay P. ¹ , Tickner Jacob ² , Kenyon Johanna J. ² , Hall Ruth M. ³ , Shneider Michael M. ⁴ , Dzhaparova Alina A. ¹ , Shashkov Alexander S. ¹ , Chizhov Alexander O. ¹ , Popova Anastasiya V. ⁵ , Knirel Yuriy A. ¹

Организации

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
2	Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, QLD 4059, Australia
3	School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia
4	M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
5	State Research Center for Applied Microbiology and Biotechnology, Obolensk, 142279 Moscow Region, Russia

Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1→3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes α l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated. Keywords: Acinetobacter ba

Kasimova A.A. , Arbatsky N.P. , Tickner J. , Kenyon J.J. , Hall R.M. , Shneider M.M. , Dzhaparova A.A. , Shashkov A.S. , Chizhov A.O. , Popova A.V. , Knirel Y.A.
Acinetobacter baumannii K106 and K112: Two Structurally and Genetically Related 6-Deoxy-l-talose-Containing Capsular Polysaccharides
International Journal of Molecular Sciences. 2021. V.22. N11. 5641 . DOI: 10.3390/ijms22115641 WOS Scopus OpenAlex

Web of science:	WOS:000660132600001
Scopus:	2-s2.0-85106441795
OpenAlex:	W3165808681

БД	Цитирований
OpenAlex	10
Scopus	11
Web of science	8