Structures of a unique O-polysaccharide of Edwardsiella tarda PCM 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing O-polysaccharide shared by E. tarda PCM 1145, PCM 1151 and PCM 1158

Structures of a unique O-polysaccharide of Edwardsiella tarda PCM 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing O-polysaccharide shared by E. tarda PCM 1145, PCM 1151 and PCM 1158 Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2012, Volume: 355, Pages: 56-62 Pages count : 7 DOI: 10.1016/j.carres.2012.04.004

Authors

Katzenellenbogen Ewa ¹ , Kocharova Nina A. ² , Toukach Philip V. ² , Górska Sabina ¹ , Bogulska Maria ¹ , Gamian Andrzej ^3,1 , Knirel Yuriy A. ²

Affiliations

1	L. Hirszfeld Institute of Immunology and Experimental, Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow, 119991, Russian Federation.
3	Department of Medical Biochemistry, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland

Lipopolysaccharides of four strains of Edwardsiella tarda were degraded by mild acid hydrolysis, and the released O-polysaccharides were isolated by GPC and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including 2D 1H, 1H COSY, TOCSY, ROESY, 1H, 13C HMBC, HSQC and HSQC-TOCSY experiments. The O-polysaccharide from E. tarda PCM 1153 was found to contain d-GalA, d-GlcNAc, d-Gal and 2-amino-1,3-propanediol (GroN). In the tetrasaccharide repeating unit, GroN is amide-linked to one of the GalA residues, and Gal is non-stoichiometrically 2- or 3-O-acetylated (∼45% at each position): image Three other E. tarda strains examined (PCM 1145, PCM 1151 and PCM 1158) share the following O-polysaccharide structure: image where Abe indicates 3,6-dideoxy-d-xylo-hexose (abequose). This structure resembles those of Citrobacter freundii O22 (PCM 1555) and Salmonella enterica O4. In accordance with the structural data, SDS–PAGE and immunoblotting of the lipopolysaccharides with anti-C. freundii O22 serum demonstrated that the O-antigens of the three E. tarda strains are serologically identical to each other and to the O-antigens of C. freundii O22 and S. enterica O4.

Katzenellenbogen E. , Kocharova N.A. , Toukach P.V. , Górska S. , Bogulska M. , Gamian A. , Knirel Y.A.
Structures of a unique O-polysaccharide of Edwardsiella tarda PCM 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing O-polysaccharide shared by E. tarda PCM 1145, PCM 1151 and PCM 1158
Carbohydrate Research. 2012. V.355. P.56-62. DOI: 10.1016/j.carres.2012.04.004 WOS Scopus OpenAlex

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