Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555

Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555 Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2009, Volume: 344, Number: 13, Pages: 1724-1728 Pages count : 5 DOI: 10.1016/j.carres.2009.06.005

Authors

Katzenellenbogen Ewa ¹ , Kocharova Nina A. ² , Toukach Philip V. ² , Górska Sabina ¹ , Korzeniowska-Kowal Agnieszka ¹ , Bogulska Maria ¹ , Gamian Andrzej ^3,1 , Knirel Yuriy A. ²

Affiliations

1	L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, Moscow 117913, Russian Federation
3	Department of Medical Biochemistry, Wrocław Medical University, Chałubińskiego 10, 50-368 Wrocław, Poland

The lipopolysaccharide of Citrobacter freundii O22 (strain PCM 1555) was degraded under mild acidic conditions and the O-polysaccharide released was isolated by gel chromatography. Sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H ROESY and 1H,13C HMBC experiments, showed that the repeating unit of the O-polysaccharide has the following structure: image where Abe is abequose (3,6-dideoxy-d-xylo-hexose). SDS–PAGE and immunoblotting revealed that the O-antigen of C. freundii O22 is serologically indistinguishable from those of Salmonella group B serovars (Typhimurium, Brandenburg, Sandiego, Paratyphi B) but not related to other abequose-containing O-antigens tested (Citrobacter werkmanii O38 and Salmonella Kentucky) or colitose (l enantiomer of abequose)-containing O-antigen of Escherichia coli O111.

Katzenellenbogen E. , Kocharova N.A. , Toukach P.V. , Górska S. , Korzeniowska-Kowal A. , Bogulska M. , Gamian A. , Knirel Y.A.
Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555
Carbohydrate Research. 2009. V.344. N13. P.1724-1728. DOI: 10.1016/j.carres.2009.06.005 WOS Scopus OpenAlex

≡ Web of science:	WOS:000269991600019
≡ Scopus:	2-s2.0-68949185019
≡ OpenAlex:	W2003882041