New structures of the O-specific polysaccharides of bacteria of the genus Proteus. 1. Phosphate-containing polysaccharides.

New structures of the O-specific polysaccharides of bacteria of the genus Proteus. 1. Phosphate-containing polysaccharides. Full article

Journal

Biochemistry-Moscow
ISSN: 0006-2979 , E-ISSN: 1608-3040

Output data

Year: 2002, Volume: 67, Number: 2, Pages: 265-276 Pages count : 12 DOI: 10.1023/a:1014482518488

Authors

Toukach F.V. ¹ , Kondakova A.N. ¹ , Arbatsky N.P. ¹ , Senchenkova S.N. ¹ , Shashkov A.S. ¹ , Knirel Y.A. ¹ , Zych K. ² , Rozalski A. ² , Sidorczyk Z. ²

Affiliations

1	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
2	Institute of Microbiology and Immunology, University of Lodz, Lodz, Poland

The O-specific polysaccharide chains (O-antigens) of the lipopolysaccharides of five Proteus strains, P. vulgaris O17, P. mirabilis O16 and O33, and P. penneri 31 and 103, were found to contain phosphate groups that link the non sugar components, e.g., ethanolamine and ribitol. The polysaccharides of P. mirabilis O16 and P. penneri 103 include ribitol phosphate in the main chain and thus resemble ribitol teichoic acids of Gram-positive bacteria. The structures of the polysaccharides were elucidated using NMR spectroscopy, including two-dimensional 1H, 1H correlation spectroscopy (COSY and TOCSY), nuclear Overhauser effect spectroscopy (NOESY or ROESY), and H-detected 1H, 13C and 1H, 31P heteronuclear multiple-quantum coherence spectroscopy (HMQC), along with chemical methods. The structures determined are unique among the bacterial polysaccharides and, together with the data obtained earlier, represent the chemical basic for classification of Proteus strains. Based on structural similarities of the O-specific polysaccharides and serological relationships between the O-antigens, we propose to extend Proteus serogroups O17 and O19 by including P. penneri strains 16 and 31, respectively.

Toukach F.V. , Kondakova A.N. , Arbatsky N.P. , Senchenkova S.N. , Shashkov A.S. , Knirel Y.A. , Zych K. , Rozalski A. , Sidorczyk Z.
New structures of the O-specific polysaccharides of bacteria of the genus Proteus. 1. Phosphate-containing polysaccharides.
Biochemistry-Moscow. 2002. V.67. N2. P.265-276. DOI: 10.1023/a:1014482518488 WOS Scopus OpenAlex

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