Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates

Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2002, Volume: 337, Number: 17, Pages: 1535-1540 Pages count : 6 DOI: 10.1016/s0008-6215(02)00265-3

Authors

Drzewiecka Dominika ¹ , Toukach Philip V ² , Arbatsky Nikolay P ² , Zych Krystyna ¹ , Shashkov Alexander S ² , Knirel Yuriy A ² , Sidorczyk Zygmunt ¹

Affiliations

1	Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódz, 90-237 Łódz, Poland
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation

The O-specific polysaccharide of the lipopolysaccharide of Proteus penneri strain 103 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H,13C HMQC, 1H, 31P HMQC, and HMBC experiments. It was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of Gram-positive bacteria. The following structure of the polysaccharide was established, where Etn and Rib-ol are ethanolamine and ribitol, respectively. This structure is unique among the known structures of Proteus O-antigens and, therefore, we propose classification of the strain studied into a new Proteus serogroup, O73. The molecular basis for cross-reactivity between O-antiserum against P. penneri 103 and O-antigens of P. mirabilis O33 and D52 is discussed.

Drzewiecka D. , Toukach P.V. , Arbatsky N.P. , Zych K. , Shashkov A.S. , Knirel Y.A. , Sidorczyk Z.
Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates
Carbohydrate Research. 2002. V.337. N17. P.1535-1540. DOI: 10.1016/s0008-6215(02)00265-3 WOS Scopus OpenAlex

≡ Web of science:	WOS:000178470600004
≡ Scopus:	2-s2.0-0037184278
≡ OpenAlex:	W1982794636