Structural and serological relatedness of the O‐antigensof Proteus penneri 1 and 4 from a novel Proteus serogroup O72

Structural and serological relatedness of the O‐antigensof Proteus penneri 1 and 4 from a novel Proteus serogroup O72 Full article

Journal

European Journal of Biochemistry
ISSN: 0014-2956 , E-ISSN: 0945-5795

Output data

Year: 2002, Volume: 269, Number: 1, Pages: 358-363 Pages count : 6 DOI: 10.1046/j.0014-2956.2001.02660.x

Authors

Sidorczyk Zygmunt ¹ , Toukach Filip V. ² , Zych Krystyna ¹ , Drzewiecka Dominika ¹ , Arbatsky Nikolay P. ² , Shashkov Alexander S. ² , Knirel Yuriy A. ²

Affiliations

1	Department of General Microbiology, Institute of Microbiology and Immunology, University of Łodź, Poland
2	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation

O-specific polysaccharides (O-antigens) of the lipopolysaccharides (LPS) of Proteus penneri strains 1 and 4 were studied using sugar analysis, 1H and 13C NMR␣spectroscopy, including 2D COSY, H-detected 1H,13C␣HMQC, and rotating-frame NOE spectroscopy (ROESY). The following structures of the tetrasaccharide (strain 1) and pentasaccharide (strain 4) repeating units of the polysaccharides were established: In the polysaccharide of P. penneri strain 4, glycosylation with the lateral Glc residue (75%) and O-acetylation of the lateral GalNAc residue (55%) are nonstoichiometric. This polysaccharide contains also other, minor O-acetyl groups, whose positions were not determined. The structural similarity of the O-specific polysaccharides was consistent with the close serological relatedness of the LPS, which was demonstrated by immunochemical studies with O-antisera against P. penneri 1 and 4. Based on these data, it was proposed to classify P. penneri strains 1 and 4 into a new Proteus serogroup, O72, as two subgroups, O72a and O72a,b, respectively. Serological cross-reactivity of P. penneri 1 O-antiserum with the LPS of P. penneri 40 and 41 was substantiated by the presence of an epitope(s) on the LPS core region shared by all P. penneri strains studied.

Sidorczyk Z. , Toukach F.V. , Zych K. , Drzewiecka D. , Arbatsky N.P. , Shashkov A.S. , Knirel Y.A.
Structural and serological relatedness of the O‐antigensof Proteus penneri 1 and 4 from a novel Proteus serogroup O72
European Journal of Biochemistry. 2002. V.269. N1. P.358-363. DOI: 10.1046/j.0014-2956.2001.02660.x OpenAlex

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