Structure of the O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Providencia alcalifaciens O32 containing N-acetylisomuramic acid

Structure of the O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Providencia alcalifaciens O32 containing N-acetylisomuramic acid Full article

Journal

Carbohydrate Research
ISSN: 1873-426X , E-ISSN: 0008-6215

Output data

Year: 2007, Volume: 342, Number: 2, Pages: 268-273 Pages count : 6 DOI: 10.1016/j.carres.2006.11.027

Authors

Bushmarinov Ivan S. ¹ , Ovchinnikova Olga G. ¹ , Kocharova Nina A. ¹ , Toukach Filip V. ¹ , Torzewska Agnieszka ² , Shashkov Alexander S. ¹ , Knirel Yuriy A. ¹ , Rozalski Antoni ²

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
2	Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, PL 90-237 Lodz, Poland

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O32 and studied by sugar and methylation analyses, solvolysis with triflic acid, 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H COSY, TOCSY, ROESY, H-detected 1H,13C HSQC and HMBC experiments. It was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose (d-GlcNAc3Slac, N-acetylisomuramic acid) with the following structure. Serological studies with O-antisera showed antigenic relationships between P. alcalifaciens O32 and O29 as well as several other Providencia and Proteus strains sharing putative epitopes on the O-polysaccharides.

Bushmarinov I.S. , Ovchinnikova O.G. , Kocharova N.A. , Toukach F.V. , Torzewska A. , Shashkov A.S. , Knirel Y.A. , Rozalski A.
Structure of the O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Providencia alcalifaciens O32 containing N-acetylisomuramic acid
Carbohydrate Research. 2007. V.342. N2. P.268-273. DOI: 10.1016/j.carres.2006.11.027 WOS Scopus OpenAlex

≡ Web of science:	WOS:000244005600013
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