Structure of the O‐specific polysaccharide of a serologically separate strain Proteus penneri 2 from a new proposed serogroup O66

Structure of the O‐specific polysaccharide of a serologically separate strain Proteus penneri 2 from a new proposed serogroup O66 Full article

Journal

European Journal of Biochemistry
ISSN: 0014-2956 , E-ISSN: 0945-5795

Output data

Year: 1999, Volume: 261, Number: 2, Pages: 392-397 Pages count : 6 DOI: 10.1046/j.1432-1327.1999.00250.x

Authors

Arbatsky Nikolay P. ¹ , Shashkov Alexander S. ¹ , Toukach Filip V. ¹ , Moll Hermann ² , Zych Krystyna ³ , Knirel Yuriy A. ¹ , Zähringer Ulrich ² , Sidorczyk Zygmunt ³

Affiliations

1	N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia;
2	Research Center Borstel, Center for Medicine and Biosciences, Germany
3	Institute of Microbiology and Immunology, University of Lodz, Poland

O-specific polysaccharide chain of Proteus penneri strain 2 lipopolysaccharide was studied by full and partial acid hydrolysis, Smith degradation, methylation analysis, and NMR spectroscopy, including two-dimensional rotating-frame NOE spectroscopy (ROESY) and 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments. Together with d-glucose and 2-acetamido-2-deoxy-d-glucose, the polysaccharide was found to contain two rarely occurring sugars, 6-deoxy-l-talose (L-6dTal) and 2,3-diacetamido-2,3,6-trideoxy-l-mannose (L-RhaNAc3NAc), and the following structure of a non-stoichiometrically O-acetylated tetrasaccharide repeating unit was established. The O-specific polysaccharide studied has a unique composition and structure and, accordingly, P. penneri 2 is serologically separate among Proteus strains. Therefore, we propose for P. penneri 2 a new Proteus O-serogroup O66 where this strain is at present the single representative.

Arbatsky N.P. , Shashkov A.S. , Toukach F.V. , Moll H. , Zych K. , Knirel Y.A. , Zähringer U. , Sidorczyk Z.
Structure of the O‐specific polysaccharide of a serologically separate strain Proteus penneri 2 from a new proposed serogroup O66
European Journal of Biochemistry. 1999. V.261. N2. P.392-397. DOI: 10.1046/j.1432-1327.1999.00250.x OpenAlex

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