New structures of the O-specific polysaccharides of Proteus. 3. Polysaccharides containing non-carbohydrate organic acids.

New structures of the O-specific polysaccharides of Proteus. 3. Polysaccharides containing non-carbohydrate organic acids. Full article

Journal

Biochemistry-Moscow
ISSN: 0006-2979 , E-ISSN: 1608-3040

Output data

Year: 2003, Volume: 68, Number: 4, Pages: 446-457 Pages count : 12 DOI: 10.1023/a:1023608231745

Authors

Kondakova A N ¹ , Toukach F V ¹ , Senchenkova S N ¹ , Arbatsky N P ¹ , Shashkov A S ¹ , Knirel Y A ¹ , Bartodziejska B ² , Zych K ² , Rozalski A ² , Sidorczyk Z ²

Affiliations

1	Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 119991, Russia.
2	Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, 90237 Lodz, Poland;

Four new Proteus O-specific polysaccharides were isolated by mild acid degradation from the lipopolysaccharides of P. penneri 28 (1), P. vulgaris O44 (2), P. mirabilis G1 (O3) (3), and P. myxofaciens (4), and their structures were elucidated using NMR spectroscopy and chemical methods. They were found to contain non-carbohydrate organic acids, including ether-linked lactic acid and amide-linked amino acids, and the following structures of the repeating units were established: →3)-α-L-QuipNAc-(1→3)-α-D-GlcpNAc-(1→6)-α-D-GlcpNAc-(1→ (S)-Lac-(2–3)⌋ (1) →4)-β-D-GlcpA-(1→3)-β-D-GalpNAc-(1→4)-β-D-Glcp-(1→3)-α-D-Galp-(1→4)-β-D-GalpNAc-(1→ L-Ala-(2–6)⌋ (2) →3)-β-D-GalpNAc-(1→6)-β-D-GalpNAc-(1→4)-β-D-GlcpA-(1→ L-Lys-(2–6)-α-D-GalpA-(1→4)⌋ (3) →4)-β-D-GlcpA-(1→6)-α-D-GalpNAc-(1→6)-β-D-GlcpNAc-(1→3)-β-D-GlcpNAc-(1→ (R)-aLys-(2–6)⌋ (4) where (S)-Lac and (R)-aLys stand for (S)-1-carboxyethyl (residue of lactic acid) and Nε-[(R)-1-carboxyethyl]-L-lysine (“alaninolysine”), respectively. The data obtained in this work and earlier serve as the chemical basis for classification of the bacteria Proteus.

Kondakova A.N. , Toukach F.V. , Senchenkova S.N. , Arbatsky N.P. , Shashkov A.S. , Knirel Y.A. , Bartodziejska B. , Zych K. , Rozalski A. , Sidorczyk Z.
New structures of the O-specific polysaccharides of Proteus. 3. Polysaccharides containing non-carbohydrate organic acids.
Biochemistry-Moscow. 2003. V.68. N4. P.446-457. DOI: 10.1023/a:1023608231745 WOS Scopus OpenAlex

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