Structural and serological studies of the O‐specific polysaccharide of the bacterium Proteus mirabilis O10 containing L‐altruronic acid, a new component of O‐antigens

Structural and serological studies of the O‐specific polysaccharide of the bacterium Proteus mirabilis O10 containing L‐altruronic acid, a new component of O‐antigens Full article

Journal

FEBS Letters
ISSN: 0014-5793 , E-ISSN: 1873-3468

Output data

Year: 1996, Volume: 398, Number: 2-3, Pages: 297-302 Pages count : 6 DOI: 10.1016/s0014-5793(96)01061-7

Authors

St. Swierzko Anna ¹ , Shashkov Alexander S. ² , Senchenkova Sof'ya N. ² , Toukach Filip V. ² , Ziolkowski Andrzej ¹ , Cedzynski Maciej ¹ , Paramonov Nikolay A. ² , Kaca W. ¹ , Knirel Yuriy A. ²

Affiliations

1	Center of Microbiology and Virology, Polish Academy of Sciences, ul. Lodowa 106, 93–232 Lodz, Poland
2	N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 117913 Moscow, Russia

An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid, and l-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral l-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.

St. Swierzko A. , Shashkov A.S. , Senchenkova S.N. , Toukach F.V. , Ziolkowski A. , Cedzynski M. , Paramonov N.A. , Kaca W. , Knirel Y.A.
Structural and serological studies of the O‐specific polysaccharide of the bacterium Proteus mirabilis O10 containing L‐altruronic acid, a new component of O‐antigens
FEBS Letters. 1996. V.398. N2-3. P.297-302. DOI: 10.1016/s0014-5793(96)01061-7 Scopus OpenAlex

≡ Scopus:	2-s2.0-0030566813
≡ OpenAlex:	W1981847201